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ProteomicsNonionic surfactants can modify the thermal stability of globular and membrane proteins interfering with the thermal proteome profiling principles to identify protein targets
The membrane proteins are essential targets to understand cellular function. The unbiased identification of membrane protein targets is still the bottleneck for a system- level understanding of cellular response to stimuli or perturbations. It has been suggested to enrich the soluble proteome with m...
ORGANISM(S): Rattus norvegicus (Rat) 
2023-03-20 | PXD037153 | Pride
Rat liver tissue Igepal Micelles Nonionic surfactants Thermal proteome profiling Nonidet-p40 substitute 
ProteomicsIdentifying Functionally Relevant Proteins and Phosphorylation Sites During T Cell Activation Using an Integrated Thermal Shift Assay
In this study, we sought to identify how protein thermal stability integrates with changes in cell signaling and protein abundance. We developed a pipeline to investigate changes in protein abundance and thermal stabilite by combining the protein abundance and thermal shift measurements into the sam...
ORGANISM(S): Mus musculus (Mouse) 
2024-06-17 | PXD050772 | Pride
Phosphoproteomics T cell activation Thermal stability 
ProteomicsInvestigation of cellular response to the HSP90 inhibition in human cells through thermal proteome profiling
Heat shock proteins are responsible for protein folding in cells. HSP90 is one of the most important chaperones in human cells, and inhibiting HSP90 is a potential strategy for cancer therapy. Multiple HSP90 inhibitors have been in clinical trials. However, none of them has been approved for disease...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD037575 | Pride
Heat shock protein 90 Hsp90 inhibitors And protein abundance change Thermal proteome profiling Cell response Protein thermal stability shift 
ProteomicsLarge-scale characterization of drug mechanism of action using proteome-wide thermal shift assays - Revisions
In response to an ever-increasing demand of new small molecules therapeutics, numerous chemical and genetic tools have been developed to interrogate compound mechanism of action. Owing to its ability to characterize compound-dependent changes in thermal stability, the proteome-wide thermal shift ass...
ORGANISM(S): Homo sapiens (Human) 
2025-02-13 | PXD053138 | Pride
Thermal shift assay Proteomics Drug discovery 
UnknownQuantitation of protein-protein interactions by thermal stability shift analysis.
Not available
S-EPMC3189529 | biostudies-literature
ProteomicsLarge-scale characterization of drug-induced changes in thermal stability using proteome-wide thermal shift assays
In response to an ever-increasing demand of new small molecules therapeutics, numerous chemical and genetic tools have been developed to interrogate compound mechanism of action. Owing to its ability to characterize compound-dependent changes in thermal stability, the proteome-wide thermal shift ass...
ORGANISM(S): Homo sapiens (Human) 
2025-02-13 | PXD048009 | Pride
Mechanism of action Drug development 
ProteomicsProteome-wide cellular thermal shift assay reveals novel crosstalk between brassinosteroid and auxin signaling
Despite the growing interest in using chemical genetics in plant research, small-molecule target identification remains a major challenge. The cellular thermal shift assay coupled with high-resolution massspectrometry (CETSA MS) that monitors changes in the thermal stability of proteins caused by th...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-04-04 | PXD030309 | Pride
Brassinosteroids Auxin Chemical genetics Cellular thermal shift assay 
UnknownAnalysis of protein stability and ligand interactions by thermal shift assay.
Not available
S-EPMC4332540 | biostudies-literature
UnknownOptimization of protein samples for NMR using thermal shift assays.
Not available
S-EPMC4869703 | biostudies-literature
ProteomicsMitochondrial dysfunction rapidly modulates the abundance and thermal stability of cellular proteins
Cellular functionality relies on a well-balanced, but highly dynamic proteome. Dysfunctionof mitochondrial protein import leads to the cytosolic accumulation of mitochondrial precursor proteins which compromise cellular proteostasis and trigger the mitoprotein-induced stress response. To dissect the...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-02-21 | PXD037741 | Pride
Mitochondria / proteasome / protein folding / proteostasis / thermal proteome profiling / stress response 
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