Sort   by:  
 Page size 
UnknownEffect of Different Proteinase K Digest Protocols and Deparaffinization Methods on Yield and Integrity of DNA Extracted From Formalin-fixed, Paraffin-embedded Tissue.
Not available
S-EPMC7045302 | biostudies-literature
ProteomicsProteomics Using Proteases Alternative to Trypsin Benefits from Sequential Digestion with Trypsin
High specificity and ease of use make trypsin the most used enzyme in proteomics. Proteases with complementary cleavage specificity to trypsin have been applied to obtain additional data. However, use of proteases with broad specificity proved especially challenging. In this work, we analyzed the ch...
ORGANISM(S): Schizosaccharomyces pombe OY26 
2021-06-07 | PXD017321 | Pride
Alternative proteases Sequential digest 
ProteomicsAutophagosome content profiling - STAR Protocol Data Set
The ascorbate peroxidase APEX2 is commonly used to study the neighborhood of a protein of interest by proximity-dependent biotinylation. Here, we describe a protocol for sample processing compatible with immunoblotting and mass spectrometry that is suitable to specifically map the content of autopha...
ORGANISM(S): Homo sapiens (Human) 
2021-04-06 | PXD024335 | Pride
Human Apex2 Proximity proteomics Proteinase k digest 
TranscriptomicsArray comparative genomic hybridization analysis of flat epithelial atypia (DIN1a) and lobular intraepithelial neoplasia
Low grade flat ductal intraepithelial neoplasia (DIN1a, flat epithelial atypia) is one of the earliest morphologically recognizable neoplastic lesions of the breast. Frequently, it occurs in association with lobular intraepithelial neoplasia (LIN). The aim of this study was to elucidate chromosomal ...
ORGANISM(S): Homo sapiens 
2010-05-05 | E-GEOD-18187 | biostudies-arrayexpress
TranscriptomicsPositioning of Necrotic Lobular Intraepithelial Neoplasias within the sequence of breast carcinoma progression
Lobular intraepithelial neoplasia grade 3 (LIN) is a recently recognized variant of intraepithelial lobular neoplasia of the breast composed of either uniform, generally small, cells (classic) with massive lobular distension, pleomorphic cells, signet-ring cells, or any cell type with necrosis. In ...
ORGANISM(S): Homo sapiens 
2010-06-20 | E-GEOD-14803 | biostudies-arrayexpress
OtherExplored the underlying mechanism by which ILF3 exerted carcinogenesis and its potential targets in MM
An RIP experiment was performed in ARP1 cell following the instructions of the article. ~5-20 × 10^6 cells were harvested and lysed. Protein A/G MagBeads pre-coated with 5μg of the antibody of interest (HNRNPA2B1/ILF3, Proteintech) and incubated with cell lysate supernatant overnight at 4°C.The bead...
ORGANISM(S): Homo sapiens 
2021-11-30 | GSE162303 | GEO
OtherThe underlying mechanism by which SFRS8 exerted carcinogenesis and its potential targets in MM
An RIP experiment was performed in ARP1 cell following the instructions of the article.~5-20 × 10e6 cells were harvested and lysed. Protein A/G MagBeads pre-coated with 5μg of the antibody of interest (SFRS8, abcam) and incubated with cell lysate supernatant overnight at 4°C.The beads containing imm...
ORGANISM(S): Homo sapiens 
2022-12-31 | GSE174823 | GEO
GenomicsNucleosome mapping of Tetrahymena thermophila chromatin in vivo and in vitro
A conserved hallmark of eukaryotic chromatin architecture is the distinctive array of well-positioned nucleosomes downstream of transcription start sites (TSS). Recent studies indicate that trans-acting factors establish this stereotypical array. Here, we present the first genome-wide in vitro and i...
ORGANISM(S): Tetrahymena thermophila 
2015-07-30 | E-GEOD-64061 | biostudies-arrayexpress
ProteomicsTissue protease activity with MALDI MSI
Aberrant protease activity has been implicated in the etiology of various prevalent diseases including neurodegeneration and cancer, in particular metastasis. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) has recently been established as a key technology for bio...
ORGANISM(S): Bos Taurus (ncbitaxon:9913) Mus Musculus (ncbitaxon:10090) 
MSV000086102 | MassIVE
TranscriptomicsRNA-binding protein AUF1 promotes myogenesis by regulating MEF2C expression levels
The mammalian RNA-binding protein AUF1 (AU-binding factor 1, also known as heterogeneous nuclear ribonucleoprotein D, hnRNP D) binds to numerous mRNAs and influences their post-transcriptional fate. Given that many AUF1 target mRNAs encode muscle-specific factors, we investigated the function of AU...
ORGANISM(S): Mus musculus 
2014-12-22 | E-GEOD-58259 | biostudies-arrayexpress
Sort   by:  
 Page size