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MetabolomicsMetaboKit: a comprehensive data extraction tool for untargeted metabolomics (Standard mixture assay)

We have developed MetaboKit, a comprehensive software package for compound identification and relative quantification in mass spectrometry-based untargeted metabolomics analysis. In data dependent acquisition (DDA) analysis, MetaboKit constructs a customized spectral library with compound identit...

2021-11-05 | MTBLS1311 | MetaboLights
MetabolomicsProMetIS, deep phenotyping of mouse models by combined proteomics and metabolomics analysis

Genes are pleiotropic and getting a better knowledge of their function requires a comprehensive characterization of their mutants. Here, we generated multi-level data combining phenomic, proteomic and metabolomic acquisitions from plasma and liver tissues of two C57BL/6 N mouse models lacking the...

2021-09-01 | MTBLS1903 | MetaboLights
MetabolomicsProteomics and tracer metabolomics link GAPDH ISGylation to glycolytic control

Ubiquitin-like protein ISG15 (interferon-stimulated gene 15) has been implicated in the regulation of central carbon metabolism, but conflicting findings across experimental systems have limited mechanistic insight. Here, we applied a multi-omics approach in cells ectopically expressing the ISGyl...

2026-03-02 | MTBLS12619 | MetaboLights
MetabolomicsAn Integrated Biochemical, Proteomics, and Metabolomics Approach for Supporting Medicinal Value of Panax ginseng Fruits (GC-MS assay)
Panax ginseng roots are well known for their medicinal properties and have been used in Korean and Chinese traditional medicines for 1000s of years. However, the medicinal value of P. ginseng fruits remain poorly characterized. Using gas chromatography-time of flight mass spectrometry (GC-TOF MS), 6...
2016-11-18 | MTBLS350 | MetaboLights
ProteomicsA multi-omics dataset of human transcriptome and proteome stable reference
In recent years, the development of high-throughput omics technology has greatly promoted the development of biomedicine. However, the poor reproducibility of omics techniques limits its application. It is necessary to use standard reference materials of complex RNAs or proteins to test and calibrat...
ORGANISM(S): Homo Sapiens 
PXD041292 |
ProteomicsEvaluation of NCI-7 Cell Line Panel
Reference materials are vital to benchmarking the reproducibility of clinical tests and essential for monitoring laboratory performance for clinical proteomics. The reference material utilized for mass spectrometric analysis of the human proteome would ideally contain enough proteins to be suitably ...
ORGANISM(S): Homo sapiens (Human) 
2018-05-14 | PXD008952 | Pride
Nci-60 Proteomics reference standard Nci-7 Cptac 
ProteomicsLarge-Scale Targeted Proteomics Using Internal Standard Triggered-Parallel Reaction Monitoring
Development, implementation, and evaluation of a new data acquisition scheme called internal standard triggered-parallel reaction monitoring (IS-PRM) to increase the scale of targeted quantitative experiments while retaining high detection and quantification performance. All the details about the da...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080832 | MassIVE
ProteomicsQuartet Protein Reference Materials for Multi-platform Assessment of Label-free Proteomics
We developed the Quartet as a bulk of proteome reference materials with built-in truth, and distributed the same aliquots to 15 laboratories in six cities of China with nine conventional LC-MS/MS platforms. Relative protein abundances from over 12,000 proteins on 816 MS files were obtained and compa...
ORGANISM(S): Homo Sapiens 
PXD043262 |
ProteomicsProbing the Sensitivity of the Lumos Mass Spectrometer using a Standard Reference Protein in a Complex Background
we determined the sensitivity of the Lumos mass spectrometer using the NIST monoclonal antibody reference material at various concentrations to detect its peptides in a background of S. cerevisiae whole cell lysate, which was kept at a constant concentration.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-09-12 | PXD009441 | Pride
Data-dependent acquisition Orbitrap lumos Mass spectrometry Proteomics Limit of detection Sensitivity 
ProteomicsReproducible protein quantitation of 270 human proteins at increased depth using nanoparticle-based fractionation and reaction monitoring mass spectrometry with stable isotope-labelled internal standards
When using a mix of 274 light synthetic peptide standards as surrogates for 270 human plasma proteins, as well as stable isotope-labelled standards as normalizers for targeted quantitative analysis by LC/MRM-MS, the Seer Proteograph allowed for the enrichment and absolute quantitation of up to an ad...
ORGANISM(S): Homo Sapiens 
2025-02-17 | PXD053704 | panorama
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