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Proximity-based proteomics (BioID) uncover Rho GTPase -interactome in kidney podocytes.
APEX2-mediated proximity labeling pioneers in situ capture of spatiotemporal protein complexes in living cells but is limited by its high background. Here, we developed an optimized probe BP5 with much lower labeling noise. Combining the new proximity labeling with label-free quantitative proteomics...
ORGANISM(S): Homo sapiens (Human) 
2020-11-18 | PXD020709 | Pride
Proximity labeling approaches have been widely utilized to define protein interactomes. Due to the inherent promiscuity of proximity labeling using TurboID-based approaches, identification and adoption of appropriate labeling controls is a pivotal step to mitigate background interference and enhance...
ORGANISM(S): Homo sapiens (Human) 
2025-07-14 | PXD061564 | Pride
Proximity labeling approaches have been widely utilized to define protein interactomes. Due to the inherent promiscuity of proximity labeling using TurboID-based approaches, identification and adoption of appropriate labeling controls is a pivotal step to mitigate background interference and enhance...
ORGANISM(S): Homo sapiens (Human) 
2025-07-14 | PXD061540 | Pride
Proximity labeling approaches have been widely utilized to define protein interactomes. Due to the inherent promiscuity of proximity labeling using TurboID-based approaches, identification and adoption of appropriate labeling controls is a pivotal step to mitigate background interference and enhance...
ORGANISM(S): Homo sapiens (Human) 
2025-07-14 | PXD061566 | Pride
Proximity labeling approaches have been widely utilized to define protein interactomes. Due to the inherent promiscuity of proximity labeling using TurboID-based approaches, identification and adoption of appropriate labeling controls is a pivotal step to mitigate background interference and enhance...
ORGANISM(S): Homo sapiens (Human) 
2025-07-14 | PXD061541 | Pride
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