We immuno-precipitated NDP including LXN, RPL29 and FAU, respectively, and NUP including AHSG, ALB and TF, respectively, before setting the immuno-precipitates to the Mass spectrometry (MS).
We performed proteomics isobaric tags for relative and absolute quantification (iTraq) to identify proteins that changed before and after NMT1 was knocked down in both Bel-7402 and Bel-7404 cells.