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TranscriptomicsTranscriptome analysis of compound-treated cells
Huh7.5.1 cells were incubated for 48 h with LY-411575, JNJ-1802, NITD-688, and NITD-008 at their respective EC90 concentrations against DENV: 10 nM, 0.1 nM, 7 nM, and 1.3 μM. Cells treated with DMSO served as controls. Three replicates for each treatment.Total RNA was extracted using the RNeasy Mini...
ORGANISM(S): Homo sapiens 
2024-12-29 | E-MTAB-14694 | biostudies-arrayexpress
GenomicsAmplicon Sequencing of gRNAs from Brunello CRISPR mutagenized HT29-DKO cells after live/dead selection with PeV-A1 and PeV-A2
HT29-DKO cells were stably transduced with lentiCas9-Blast (Addgene, #52962) and subsequently selected using Blasticidin. Then, 300 million HT29-DKO cells that constitutively express Cas9 were transduced with lentiGuide-Puro from the Brunello library at MOI 0.3. Cells were then selected with puromy...
ORGANISM(S): Homo sapiens 
2024-03-16 | E-MTAB-13894 | biostudies-arrayexpress
GenomicsAmplicon Sequencing of gRNAs from Druggable Genome CRISPR mutagenized Huh7.5.1 cells after live/dead selection with DENV2
Huh7.5.1 cells were stably transduced with lentiCas9-Blast (Addgene, #52962) and subsequently selected using Blasticidin. Then, Huh7.5.1 cells that constitutively express Cas9 were transduced with lentiGuide-Puro from the druggable genome library at MOI 0.3. Cells were then selected with puromycin, ...
ORGANISM(S): Homo sapiens 
2024-12-29 | E-MTAB-14693 | biostudies-arrayexpress
GenomicsAmplicon Sequencing of gRNAs from Brunello CRISPR mutagenized A549 and U87-MG cells after live/dead selection with EV-D68 IL
With the near eradication of poliovirus due to global vaccination campaigns, attention has shifted to other enteroviruses that can cause polio-like paralysis syndrome (now termed acute flaccid myelitis (AFM)). In particular, enterovirus D68 (EV-D68) is believed to be the main driver of epidemic outb...
ORGANISM(S): Homo sapiens 
2025-02-25 | E-MTAB-14765 | biostudies-arrayexpress
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