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Gene expression patterns were surveyed using RNA from 4 different murine sources. Four classes of RNA were analyzed: undifferentiated embryonic stem cells, differentiated ES cells, kidney and liver tissues. Classes were prepared in triplicate for a total of 12 samples. All samples were hybridized to...
ORGANISM(S): Mus musculus 
Quantitative PCR (qRT-PCR) analysis of micro RNA expression in mouse cells
A major virulence factor in S. enterica is the pathogenicity island Spi2. The goal of the experiment was to determine the impact of the toll-like receptor 4 (TLR-4) on expression of Spi2 in S. enterica wild type (WT) and triple mutant strain (TMS) with deletions in SPI-1 TTSS, SPI-2 TTSS, flagella....
ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 
circEPS15 overexpression in hepatocellular carcinoma modulates tumor invasion and migration [qRT-PCR validation]
A major virulence factor in S. enterica is the pathogenicity island Spi2. The goal of the experiment was to determine the impact of the NRAMP-1 (natural resistance-associated macrophage protein-1) on expression of Spi2 in S. enterica wild type (WT) and triple mutant strain (TMS) with deletions in SP...
ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 
Chelonus formosanus is an important parasitic natural enemy of Lepidopteran insects and has great value in the biological control of insect pests. To screen the reference genes in the real-time fluorescence quantitative PCR (qRT-PCR) assay of Chelonus formosanus, six candidate reference genes were s...
ORGANISM(S): Chelonus formosanus 
2024-12-14 | GSE283846 | GEO
Sequences of low copy number transcripts from sugarcane cultivars N22 and SP80-3280 identified by PCR and sequenced with MinION. These transcripts support the selecton of low copy number genes for qRT-PCR experiments.
Human tissue based proteomics projects are challenging due to low abundance of proteins and tissue specificity of protein expression. In this study, we aimed to develop a cell-based approach to profile the male specific region of the Y chromosome (MSY) proteins. First, we profiled the expression of ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-06-10 | MSV000083949 | MassIVE
We used high-throughput qRT-PCR analysis to obtain full genome qRT- PCR data for the Staphylococcus aureus strains USA300 (wild type) and TB15 (mutant). Both strains were collected during infection from 4 mouse organs ( skin, kidney, lung,liver) as well as from human neutrophil infection.
ORGANISM(S): Staphylococcus aureus 
2011-06-13 | GSE24701 | GEO
Quantitative free labeling proteomics to elucidate the role of the DapA protein in iron metabolism and the assimilation of cyanide in the bacterium Pseudomonas pseudoalcaligenes. Integration, validation and discussion of results using other non-proteomic techniques such as qRT-PCR and determination ...
ORGANISM(S): Pseudomonas pseudoalcaligenes CECT 5344 Bacteria 
2020-02-18 | PXD014027 | Pride
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