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DLD1 is an APC mut, KRAS mut, P53 mut CRC cell line. PROX1 transcription factor, target of Wnt pathway in CRC, is our protein of interest.DLD1 cells are PROX1 negative. We overexpressed through lentiviral expression PROX1 protein or the empty vector psd44, through selection of the cells in puromycin...
ORGANISM(S): Homo Sapiens 
We performed ChIP-seq data in SW480R cells. Pools of three independent ChIP experiments and input DNA were used for sequencing.
ORGANISM(S): Homo sapiens 
SW480R is an APC mut, KRAS mut, P53 mut CRC cell line. PROX1 transcription factor, target of Wnt pathway in CRC, is our protein of interest.SW480R cells are PROX1 positive. We suppressed through siRNA transfection the expression of PROX1 protein or non-targeting siRNA, using two different siRNA. Aft...
ORGANISM(S): Homo Sapiens 
PROX1 or §-catenin was depleted in SW480 by siRNA transfection and the gene expression profiles were compared to negative controls to study the genes affected.
ORGANISM(S): Homo sapiens 
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