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Transcriptional profiling of Ramos germinal center B cells, comparing untreated cells to cells treated with etoposide, and untreated cells to cells treated with anti-IgM. Both treatments, engagement of the B-cell receptor with anti-IgM and induction of DNA double-strand breaks with etoposide, resul...
ORGANISM(S): Homo sapiens 
ChIP-on-chip experiment from Ramos cells to analyze genome-wide CRTC2 binding sites in germinal center B cells. The goal was to combine information from our ChIP-on-chip and expression array analyses to compile a list of CRTC2 target genes. CRTC2 was immunoprecipitated from sheared chromatin isolat...
ORGANISM(S): Homo sapiens 
The Chromosome 16 Consortium is integrated in the Human Proteome Project that aims to develop an entire map of the proteins encoded by the human genome following a gene-centric strategy (C-HPP) to make progress in the understanding of human biology in health and disease (B/D-HPP). To do this study f...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-06-12 | MSV000083967 | MassIVE
Integrated in the Chromosomic-centric Human Proteome Project (C-HPP), a lymphocytic B-cell line (Ramos) was selected as a model of B-cells. We performed a subfractionation strategy (in triplicate) for the extraction of proteins from 4 different fractions (cytoplasm, CYT; organella, ORG; membrane, MB...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-07-20 | MSV000079936 | MassIVE
We report the application of a high-throughput technique, RNA-seq, to study the transcriptomic response of P. putida DOT-T1E in the presence of antibiotics with different mechanisms of action with the aim to study in more detail the defense mechanisms that bacteria use to resist against toxic compou...
ORGANISM(S): Pseudomonas putida DOT-T1E 
Integrated in the Chromosomic-centric Human Proteome Project (C-HPP), a lymphocytic B-cell line (Ramos) was selected as a model of B-cells. We performed a subfractionation strategy (in triplicate) for the extraction of proteins from 4 different fractions (cytoplasm, CYT; organella, ORG; membrane, MB...
ORGANISM(S): Homo sapiens (Human) 
2015-08-04 | PXD001933 | Pride
This SuperSeries is composed of the following subset Series: GSE23169: Transcriptional profiling of Ramos germinal center B cells GSE23170: ChIP-on-chip experiment from Ramos cells to analyze genome-wide CRTC2 binding sites in germinal center B cells Refer to individual Series
ORGANISM(S): Homo sapiens 
The Chromosome 16 Consortium is integrated in the Human Proteome Project that aims to develop an entire map of the proteins encoded by the human genome following a gene-centric strategy (C-HPP) to make progress in the understanding of human biology in health and disease (B/D-HPP). To do this study f...
ORGANISM(S): Homo sapiens (Human) 
2013-09-05 | PXD000447 | Pride
To uncover the chromosome 16 associated proteome and to take advantage of the generated knowledge to make progress in human biology in health and disease, a consortium of 15 groups was organized in four working groups: SRM and protein sequencing, antibody and peptide standard, clinical healthcare an...
ORGANISM(S): Homo sapiens 
It has been performed a genome-wide analysis of gene expression of the root-colonizing bacterium Pseudomonas putida KT2440 in the rhizosphere of corn (Zea mays var. Girona. To identify reliable rhizosphere differentially expressed genes, rhizosphere populations of P. putida bacteria cells were compa...
ORGANISM(S): Pseudomonas putida 
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