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MCF7 cell was an important stabilized breast cancer cell in cancer research. A membrane proteomic method was developed and compared in this study. A membrane protein enrichment method composed of ultra-centrifugation and detergent-based extraction was first developed. Then in-solution digestion with...
ORGANISM(S): Homo sapiens (Human) 
2016-10-07 | PXD004131 | Pride
Staphylococcus aureus strain ATCC 1718 was inoculated at 0.5% in 400 mL of Brain Heart Infusion broth for 8 h under aerobic conditions. The supernatant was collected by centrifugation twice at 5,000 x g, 4°C for 20 min, filtrated through a 0.45 µm filter to remove all remaining bacterial cells. S. a...
ORGANISM(S): Staphylococcus Aureus 
HDCA supplementation ameliorated diet-induced NAFLD in C57 wild type mice. In attempt to comprehensively understand the molecular mechanism occurring during the protective effect of HDCA, transcriptomic and proteomic profiles of mouse liver were first analyzed utilizing RNA-Sequencing and Tandem Mas...
ORGANISM(S): Mus Musculus 
2023-08-18 | PXD044613 |
Although the cellular concentration of miRNAs is critical to their function, how miRNA expression and abundance are regulated during ontogeny is unclear. We used deep-sequencing to characterize the microRNome from most developing lymphocytes, various hematopoietic cell lineages, and representative m...
ORGANISM(S): Mus musculus 
In autoimmune diseases, accumulation of activated leukocytes correlates with inflammation and disease progression, and therefore, disruption of leukocyte trafficking is an active area of research. The protein kinase Tpl2 (MAP3K8) regulates leukocyte inflammatory responses and is also being investig...
ORGANISM(S): Mus musculus 
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