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GenomicsGenome-wide maps of RAP1 binding sites.
By performing chromatin immunoprecipitation coupled with ultra-high-throughput sequencing (ChIP-seq), we find that RAP1 binds to telomeres as well as to extra-telomeric sites through the (TTAGGG)2 consensus motif. Extra-telomeric RAP1 binding sites are particularly abundant at subtelomeric regions, ...
ORGANISM(S): Mus musculus 
2010-07-10 | E-GEOD-20867 | biostudies-arrayexpress
TranscriptomicsMeiosis timecourse Rap1-depletion Rap1 ChIP-chip
To confirm that Rap1-depletion resulted in decreased binding of Rap1 to its target sites, we performed compared Rap1 ChIP-chip experiments in respiratory cells (pre-meiotic/YPA 20 hours) depleted (plus doxycycline for 12 hours) or not depleted (minus doxycycline) of Rap1. There is growing recognitio...
ORGANISM(S): Saccharomyces cerevisiae SK1 
2016-09-01 | E-GEOD-75260 | biostudies-arrayexpress
TranscriptomicsMeiosis timecourse Rap1-depletion expression profile
To investigate the impact of Rap1 binding on nearby gene expression during the transition from vegetative growth to meiosis, we measured RNA abundance in cells depleted (plus doxycycline) or not depleted (minus doxycycline) of Rap1. RNA abundance was measured in depleted and non-depleted condition i...
ORGANISM(S): Saccharomyces cerevisiae SK1 
2016-09-01 | E-GEOD-75259 | biostudies-arrayexpress
TranscriptomicsAbf1 and Rap1 effect on nucleosome occupancy in yeast
We grew wild type and corresponding mutant abf1 and rap1 temperature sensitive yeast at 25 C, then for one hour after shifting to 37 C. We then prepared nucleosomal (mostly mononucleosomal) DNA and hybridized to tiling arrays. Three abf1 ts mutant and corresponding wild type, and three rap1 ts muta...
ORGANISM(S): Saccharomyces cerevisiae 
2010-10-15 | E-GEOD-22514 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of yeast Rap1 and Abf1 DNA-binding temperature sensitive mutants and wild type controls
Abf1 and Rap1 are General Regulatory Factors that contribute to transcriptional activation of a large number of genes, as well as to replication, silencing, and telomere structure in yeast. In spite of their widespread roles in transcription, the scope of their functional targets genome-wide has no...
ORGANISM(S): Saccharomyces cerevisiae 
2009-04-24 | E-GEOD-6073 | biostudies-arrayexpress
TranscriptomicsRoles of mammalian Rap1 in TERT recruitment to chromatin, subtelomeric gene silencing and transcriptional regulation
Repressor-activator protein 1 (scRap1) is the major binding activity at Saccharomyces cerevisiae telomeres, with roles in telomere length regulation and establishment of subtelomeric silencing by recruiting the Sir proteins. scRap1 also acts as a transcription factor controlling the expression of ri...
ORGANISM(S): Mus musculus 
2010-07-10 | E-GEOD-19011 | biostudies-arrayexpress
TranscriptomicsRap1, Sir2, Sir3, Sir4 immunoprecipitations
All Rap1, Sir2, Sir3, Sir4, and mock immunoprecipitation experiments associated with Lieb et al. Nature Genetics, August 2001, Volume 28, Issue 4 Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Computed
ORGANISM(S): Saccharomyces cerevisiae 
2005-09-24 | E-GEOD-3360 | biostudies-arrayexpress
TranscriptomicsRAP1 protects from obesity, diabetes and metabolic syndrome through its extra telomeric role regulating gene expression (old mouse liver)
RAP1 is one of the components of shelterin, the capping complex at chromosome ends or telomeres. Unlike other shelterins, however, RAP1 is not essential for preventing telomere fusions. RAP1 also binds along chromosome arms, where it is proposed to regulate gene expression. To investigate the non-te...
ORGANISM(S): Mus musculus 
2013-06-21 | E-GEOD-43173 | biostudies-arrayexpress
TranscriptomicsRAP1 protects from obesity, diabetes and metabolic syndrome through its extra telomeric role regulating gene expression (young mouse liver)
RAP1 is one of the components of shelterin, the capping complex at chromosome ends or telomeres. Unlike other shelterins, however, RAP1 is not essential for preventing telomere fusions. RAP1 also binds along chromosome arms, where it is proposed to regulate gene expression. To investigate the non-te...
ORGANISM(S): Mus musculus 
2013-06-21 | E-GEOD-43171 | biostudies-arrayexpress
TranscriptomicsRAP1 protects from obesity, diabetes and metabolic syndrome through its extra telomeric role regulating gene expression (young mouse white fat)
RAP1 is one of the components of shelterin, the capping complex at chromosome ends or telomeres. Unlike other shelterins, however, RAP1 is not essential for preventing telomere fusions. RAP1 also binds along chromosome arms, where it is proposed to regulate gene expression. To investigate the non-te...
ORGANISM(S): Mus musculus 
2013-06-21 | E-GEOD-43172 | biostudies-arrayexpress
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