Rap1, Sir2, Sir3, Sir4 immunoprecipitations
Ontology highlight
ABSTRACT:
ORGANISM(S): Saccharomyces cerevisiae
SUBMITTER: Jason Lieb
PROVIDER: E-GEOD-3360 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Promoter-specific binding of Rap1 revealed by genome-wide maps of protein-DNA association.
Lieb J D JD Liu X X Botstein D D Brown P O PO
Nature genetics 20010801 4
We determined the distribution of repressor-activator protein 1 (Rap1) and the accessory silencing proteins Sir2, Sir3 and Sir4 in vivo on the entire yeast genome, at a resolution of 2 kb. Rap1 is central to the cellular economy during rapid growth, targeting 294 loci, about 5% of yeast genes, and participating in the activation of 37% of all RNA polymerase II initiation events in exponentially growing cells. Although the DNA sequence recognized by Rap1 is found in both coding and intergenic seq ...[more]
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