Gene expression patterns within cell lines are predictive of chemosensitivity

ABSTRACT: We generated RNA's and measured expression of 40000 genes using spotted cDNA microarrays from the fifty nine publicly available cell lines of the NCI Developmental Therapeutics Program's NCI60 studies and an additional set of seven cell lines for which GI50 compound sensitivity data were available. All cell lines were grown to 80% confluence in RPMI 1640 supplemented with phenol red, glutamine (2 mM) and 5% fetal calf serum. This expression data, in conjunction with the compound sensitivity data sets available from the DTP, were used to empirically determine whether gene-compound correlates of a sufficiently high correlation coefficient would have a suitable low false discovery rate to support the use of a correlative approach and these datasets for early discovery approaches for new targe

ORGANISM(S): Homo sapiens

SUBMITTER: Brian Ring 

PROVIDER: E-GEOD-7947 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress