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We performed microRNA sequencing (miRNA-seq) according to Illumina sequencing protocols. Total RNA extracted from MCF-7 cells expressing human ER-beta in absence of estrogen stimuli was subjected to size selection labrary preparation ond retrotranscribed. Obtained DNA was sequenced with the Illumina...
ORGANISM(S): Homo sapiens 
We performed microRNA sequencing (miRNA-seq) according to Illumina sequencing protocols. Total RNA extracted from MCF-7 cells expressing or not human Erbeta in absence of estrogen stimuli was subjected to size selection labrary preparation ond retrotranscribed. Obtained DNA was sequenced with the Il...
ORGANISM(S): Homo sapiens 
We performed microRNA sequencing (miRNA-seq) according to Illumina sequencing protocols. Total RNA extracted from ZR75.1 cells expressing or not human ER-beta in absence of estrogen stimuli was subjected to size selection library preparation and retrotranscribed. Obtained DNA was sequenced with the ...
ORGANISM(S): Homo sapiens 
The transcriptomic dose-dependent response of rat immortalized thyrocytes to two thyroid disrupting chemicals was investigated by RNA-seq experiment. PCCl3 cells were exposed for one week to different doses of ethylenethiourea (ETU) and chlorpyrifos (CPF) and to their combinations.
ORGANISM(S): Rattus norvegicus 
MCF-7 TET Off cells (MCF-7 wt) were used to produce stable clones expressing ER-beta tagged with TAP-tag at the C-term (C-TAP-ER-beta). All cells were grown in Dulbecco's modified Eagle's medium (DMEM), starved by using DMEM w/o phenol red and 5% Dextran Coated Charcoal stripped serum (DCC-FBS) for ...
ORGANISM(S): Homo sapiens 
c-jun conditional knockout mice (c-jun?li) and relative controls (c-junf/f) were treated with TCPOBOP (3mg/Kg of body weight) and were sacrificed at 0, 1, 3 and 6 hours later. Gene expression analysis was performed on total RNA pools extracted from fresh frozen livers of 3 animals per group. For e...
ORGANISM(S): Mus musculus 
Myxomas, the most common primary tumor of the heart, usually develop in the atria and consist of a myxoid matrix composed of an acid-mucopolysaccharide-rich stroma with polygonal stromal cells scattered throughout the matrix. These benign tumors, despite their rarity, are a research focus because of...
ORGANISM(S): Homo sapiens 
Myxomas, the most common primary tumor of the heart, usually develop in the atria and consist of a myxoid matrix composed of an acid-mucopolysaccharide-rich stroma with polygonal stromal cells scattered throughout the matrix. These benign tumors, despite their rarity, are a research focus because of...
ORGANISM(S): Homo sapiens 
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