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In mesenchymal-like cell migration, cells need to polarise into a protrusive front and a retracting cell body. To understand this process better, we set out to quantify the distribution of cellular proteins between protrusions and cell-body by proteomics, using MDA-MB-231 cells, a highly invasive br...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-03-10 | MSV000079570 | MassIVE
Early embryogenesis is marked by extremely rapid cell cycles that generate significant replication stress (RS), but how this stress influences cell-fate potential is not fully understood. Using mouse embryonic stem cells as a model of the inner cell mass, we show that RS quickly and persistently act...
ORGANISM(S): Mus musculus (Mouse) 
2026-09-07 | PXD071534 | Pride
The transcription factor CCCTC-binding factor (CTCF) modulates pleiotropic functions mostly related to gene expression regulation. The role of CTCF in large scale genome organization is also well established. A unifying model to explain relationships between many CTCF-mediated activities involve dir...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD022037 | Pride
Addona TA, Shi X, Keshishian H, Mani DR, Burgess M, Gillette MA, Clauser KR, Shen D, Lewis GD, Farrell LA, Fifer MA, Sabatine MS, Gerszten RE, Carr SA. Nat Biotechnol. 2011, 29(7):635-43. doi: 10.1038/nbt.1899. PMCID:PMC3366591. Discovery, AIMS, and MRM datasets Discovery_Tranche_hash: b/quYtp/Q8fY...
2013-12-19 | MSV000078513 | MassIVE
Current models imply that the FERM domain protein Merlin, encoded by the tumor suppressor NF2, inhibits mitogenic signaling at or near the plasma membrane. Here, we show that the closed, growth inhibitory form of Merlin accumulates in the nucleus, binds to the E3 ubiquitin ligase CRL4DCAF1, and supp...
ORGANISM(S): Mus musculus 
Translatome analysis by sucrose gradient centrifugation of cell lysates followed by microarray profiling of the polysomal and subpolysomal RNA fractions represents a way of both studying translational control networks and better approximating the proteomic representation of cells. It is an establish...
ORGANISM(S): Homo sapiens 
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