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MetabolomicsProtein-metabolite interactomics of carbohydrate metabolism reveal regulation of lactate dehydrogenase

Metabolic networks are interconnected and influence diverse cellular processes. The protein-metabolite interactions that mediate these networks are frequently low affinity and challenging to systematically discover. We developed mass spectrometry integrated with equilibrium dialysis for the disco...

2023-08-07 | MTBLS7237 | MetaboLights
TranscriptomicsTranscription profiling of recombinant E. coli to heat-shock and dual heat-shock recombinant protein induction
Recombinant Escherichia coli cultures are used to manufacture numerous therapeutic proteins and industrial enzymes, where many of these processes use elevated temperatures to induce recombinant protein production. The heat-shock response in wild-type E. coli has been well studied. In this study, t...
ORGANISM(S): Escherichia coli 
2009-08-25 | E-GEOD-17507 | biostudies-arrayexpress
UnknownOptimization of the intron sequences combined with the CMV promoter increases recombinant protein expression in CHO cells.
Not available
S-EPMC11779943 | biostudies-literature
GenomicsDeciphering the determinants of recombinant protein yield across the human secretome
Deciphering the determinants of recombinant protein yield across the human secretome
PRJNA938154 | ENA
TranscriptomicsTranscription profiling of yeast grown in gastric or duodenal medium to identify promoters that could be used to down-regulate genes used in the release of therapeutic proteins
The use of yeast as a delivery system is an attractive option for the oral administration of therapeutic agents. We recently developed mutants of Saccharomyces cerevisiae capable of lysis upon conditional down-regulation of the expression of the cell wall genes PKC1 and SRB1. The lysis mechanism of ...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-01 | E-MEXP-2127 | biostudies-arrayexpress
ProteomicsAnalyzes of PEGylation of a Heterogeneous Therapeutic Protein by Direct Mass Technology and Data Dependent Acquisition
Intact mass of a heterogeneous therapeutic protein was determined by Direct Mass Technology (Orbitrap based charge detection mass spectrometry), proving mono PEGylation of the biologic. Furthermore, using multiple enzyme combinations, major PEGylation sites of the protein were mapped by finetuning i...
ORGANISM(S): Recombinant Protein 
MSV000099026 | MassIVE
TranscriptomicsGlobal transcriptome response of recombinant E. coli to heat-shock and dual heat-shock recombinant protein induction
Recombinant Escherichia coli cultures are used to manufacture numerous therapeutic proteins and industrial enzymes, where many of these processes use elevated temperatures to induce recombinant protein production. The heat-shock response in wild-type E. coli has been well studied. In this study, the...
ORGANISM(S): Escherichia coli Escherichia coli K-12 
2009-08-07 | GSE17507 | GEO
TranscriptomicsRecombinant and plasma-derived Factor VIII products induce distinct splenic cytokine microenvironments in hemophilia A
To investigate the gene expression in dendritic cells after treating hemiphilic mice with HBSS, plasma derived or recombinant factor VIII Gene expression in dendritic cells were measured 24 hours post treatment Hemophilic mice with treated with either (HBSS, rFVIII or pdFVIII) 24 hours post treatmen...
ORGANISM(S): Mus musculus 
2010-05-19 | E-GEOD-15809 | biostudies-arrayexpress
ModelsIrani2015 - Genome-scale metabolic model of P.pastoris N-glycosylation
Irani2015 - Genome-scale metabolic model of P.pastoris N-glycosylation This model is described in the article: Genome-scale metabolic model of Pichia pastoris with native and humanized glycosylation of recombinant proteins. Irani ZA, Kerkhoven E, Shojaosadati SA, Nielsen J. ...
2024-10-15 | BIOMD0000001096 | BioModels
FROG 
UnknownIn situ detection of protein interactions for recombinant therapeutic enzymes.
Not available
S-EPMC7855575 | biostudies-literature
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