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ProteomicsCell line xenograft experiment
Pediatric ALL cell line 380 was injected via tail vein into NSG mice. Triplicate 380 cell samples were also prepared at the timepoint of injection and stored at -80 oC for comparative analysis with CDXs. Mice were euthanized at onset of leukemia symptoms and spleen, bone marrow and liver samples wer...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-01-20 | MSV000086727 | MassIVE
ProteomicsGlobal protein analysis of pediatric acute leukemia patients and matched xenografts
Proteomics reveal protein stability after xenotransplantation in murine models. Tryptic peptide digests from patient mononuclear cells were analyzed by Data Independent Acquisition (DIA) mass spectrometry. High-pH fractionation was performed on a pool of samples, followed by data dependent acquisiti...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-12-02 | MSV000084646 | MassIVE
ProteomicsPhosphoproteome analysis of pediatric acute leukemia patients and matched xenografts
Proteomics reveal PTM stability after xenotransplantation in murine models. Phosphopeptides enriched using magnetic FeNTA beads were analyzed by Data Independent Acquisition (DIA) mass spectrometry. High-pH fractionation was performed on a pool of samples, followed by data dependent acquisition (DDA...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-12-02 | MSV000084645 | MassIVE
ProteomicsN terminome analysis of pediatric acute leukemia patients and matched xenografts
Proteomics reveal PTM stability after xenotransplantation in murine models. N termini enriched using HUNTER technique were analyzed by Data Independent Acquisition (DIA) mass spectrometry. High-pH fractionation was performed on a pool of samples, followed by data dependent acquisition (DDA) mass spe...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-12-02 | MSV000084643 | MassIVE
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