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ProteomicsRibosome r-protein content during stationary phase
The aim of this study was to determine the fate of ribosomes and r-proteins. In this respect, SILAC (Stable Isotope Labeled Amino acids in cell Culture) based experimental approach was used (Fig. 1). E.coli cells were grown in MOPS medium supplemented with “heavy” labeled arginine (Arg10) and lysine...
ORGANISM(S): Escherichia coli 
2023-09-01 | PXD035923 | Pride
Ribosome Ribosomal proteins Proteome E.coli 
ProteomicsE.coli Ribosome protein composition
Ribosomes are essential macromolecular complexes conducting protein biosynthesis in all domains of life. Cells can have heterogeneous ribosomes, i.e. ribosomes with various ribosomal RNA (rRNA) and ribosomal protein (r-protein) composition. However, the functional importance of heterogeneous ribosom...
ORGANISM(S): Escherichia coli 
2020-10-20 | PXD018123 | Pride
Ribosome Escherichia coli Lc-ms/ms 
ProteomicsRibosome protein composition in wt and 23S+5S rRNA ribosomes
The 120-nt long 5S rRNA, is an indispensable component of cytoplasmic ribosomes in all living organisms. The functions of 5S rRNA and the reasons for its evolutionary preservation as an independent molecule remain unclear. Here we used ribosome engineering to investigate whether maintaining 5S rRNA ...
ORGANISM(S): Escherichia coli 
2020-06-18 | PXD019058 | Pride
Mass-spectrometry Ribosome Proteins 
ProteomicsR-proteins in modification deficient ribosomes - free 50S subunits
Ribosomal RNA modifications are introduced by specific enzymes during ribosome assembly in bacteria. Deletion of individual modification enzymes has a minor effect on bacterial growth, ribosome biogenesis, and translation, which has complicated the definition of the function of the enzymes and their...
ORGANISM(S): Escherichia coli 
2024-03-15 | PXD047376 | Pride
R-protein E.coli ribosome 
ProteomicsR-proteins in modification deficient ribosomes - dissociated 50S subunits from 70S
Ribosomal RNA modifications are introduced by specific enzymes during ribosome assembly in bacteria. Deletion of individual modification enzymes has a minor effect on bacterial growth, ribosome biogenesis, and translation, which has complicated the definition of the function of the enzymes and their...
ORGANISM(S): Escherichia coli 
2024-03-15 | PXD047588 | Pride
Ribosomes Silac Ribosomal proteins 
ProteomicsEscherichia coli proteome during stationary phase
The aim of this study was to determine the fate of ribosomes and r-proteins. In this respect, SILAC (Stable Isotope Labeled Amino acids in cell Culture) based experimental approach was used. E.coli cells were grown in MOPS medium supplemented with “heavy” labeled arginine (Arg10) and lysine (Lys8). ...
ORGANISM(S): Escherichia coli 
2023-10-10 | PXD035927 | Pride
E.coli Ribosomal proteins Proteome 
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