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TranscriptomicsControlling for gene expression changes in transcription factor protein networks.
The development of affinity purification technologies together with mass spectrometric analyses of the purified protein mixtures (AP-MS) has been used both to identify new protein-protein interactions and to define the subunit composition of protein complexes. Transcription factor protein interactio...
ORGANISM(S): Homo sapiens 
2014-04-24 | E-GEOD-57027 | biostudies-arrayexpress
TranscriptomicsRelA and RelB-dependent transcriptome analysis in lymphotoxin-ß receptor stimulated mouse embryonic fibroblasts
Background: Lymphotoxin signaling via the lymphotoxin-β receptor (LTβR) has been implicated in several biological processes, ranging from development of secondary lymphoid organs, maintenance of splenic tissue, host defense against pathogens, autoimmunity, and lipid homeostasis. The major transcript...
ORGANISM(S): Mus musculus 
2008-12-17 | E-GEOD-11963 | biostudies-arrayexpress
TranscriptomicsPCR array of inflammatory cytokines and receptors in mouse lungs with NF-kappaB RelA mutant in alveolar epithelial cells during LPS stimulation
A murine model of RelA mutated throughout the alveolar epithelium was generated. Mice were anesthetized and intratracheally instilled with 50M-NM-
ORGANISM(S): Mus musculus 
2012-08-03 | E-GEOD-38398 | biostudies-arrayexpress
TranscriptomicsRole of RelA:CBP/p300 interaction on RelA-activated genes
A structure-function study of NF-kB subunit RelA and coactivator CBP/p300 interaction reveals the critical role of CBP/p300 in recruitment of RelA to its target promoter site. mRNA profiles of unstimulated or stimulated with TNFa rela-/- MEF reconstituted with RelA wild type or mutants were generate...
ORGANISM(S): Mus musculus 
2013-09-09 | E-GEOD-46213 | biostudies-arrayexpress
TranscriptomicsPCR array of inflammatory cytokines and receptors in mouse lungs with NF-kappaB RelA mutant in alveolar epithelial cells during pneumococcal pneumonia
A murine model of RelA mutated throughout the alveolar epithelium was generated. Mice were anesthetized and intratracheally instilled with 106 CFU of Streptococcus pneumoniae serotype 3 into the left lung lobe. Mice were euthanized after 15 hours after instillation, and left lung lobes were collecte...
ORGANISM(S): Mus musculus 
2012-08-03 | E-GEOD-38399 | biostudies-arrayexpress
TranscriptomicsCLIP-Seq experiment of Escherichia coli RelA wild type and RelA::C289Y mutant strains to identify specific RNAs that bind RelA
In this experiment, we carried out a global analysis of specific RNAs that are bound to the RelA protein. CLIP-Seq experiment was carried out with E. coli RelA wild type and RelA::C289Y mutant strains. Libraries were prepared and high throughput sequencing was done.
ORGANISM(S): Escherichia coli K-12 
2021-09-16 | E-MTAB-10884 | biostudies-arrayexpress
TranscriptomicsEgress of mature murine regulatory T cells from the thymus requires RelA
The mechanism of egress of mature regulatory T cells (Tregs) from the thymus to the periphery remains enigmatic, as does the nature of those factors expressed in the thymic environment. Here, we examined the fate of thymic Tregs in TNFα/RelA double-knockout (TA-KO) mice, because TA-KO mice retain a...
ORGANISM(S): Mus musculus 
2015-03-04 | E-GEOD-64191 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of Legionella pneumophilaEfter RelA overexpression.
Effect of ppGpp on transcript level after RelA overexpression (45 min). The cells are constructed in our lab. They are knock-out mutants in the production of ppGpp(guanosine tetraphosphate) of Legionella, complemented with an over-expression vector containing the relA gene to express the RelA prot...
ORGANISM(S): Legionella pneumophila 
2010-04-13 | E-MTAB-222 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of Legionella pneumophila ppGpp knockout and relA over expression mutants.
Effect of ppGpp on transcript level after RelA overexpression (90 min). The cells are constructed in our lab. They are knock-out mutants in the production of ppGpp(guanosine tetraphosphate) of Legionella, complemented with an over-expression vector containing the relA gene to express the RelA prot...
ORGANISM(S): Legionella pneumophila 
2010-04-14 | E-MTAB-211 | biostudies-arrayexpress
TranscriptomicsMouse Sirt6-/- RelA+/- tissues
We harvested spleen tissue from Sirt6-/- or Sirt6-/- RelA+/- animals and then completed genome-wide microarray analysis. Total RNA was extracted with TRIzol (Invitrogen) and amplified with Amino Allyl MessageAmp II Amplification kit. Amplified age-matched wild-type tissues were used as reference RNA...
ORGANISM(S): Mus musculus 
2010-06-24 | E-GEOD-13209 | biostudies-arrayexpress
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