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ProteomicsComparison of unit resolution versus high-resolution accurate mass for parallel reaction monitoring
Parallel reaction monitoring (PRM) is an increasingly popular alternative to selected reaction monitoring (SRM) for targeted proteomics. PRM’s strengths over SRM are that it monitors all product ions in a single spectrum, thus eliminating the need to select interference-free product ions prior to da...
ORGANISM(S): Homo Sapiens 
2021-08-19 | PXD023334 | panorama
ProteomicsA novel hybrid high speed mass spectrometer allows rapid translation from biomarker candidates to targeted clinical tests using 15N labeled proteins
Recent developments in affinity binder or mass spectrometry (MS)-based plasma proteomics are now producing panels of potential biomarker candidates for diagnosis or prognosis. However, clinical validation and implementation of these biomarkers remain limited by the reliance on dated triple quadrupol...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-06-17 | MSV000095051 | MassIVE
ProteomicsHighly Multiplex Targeted Proteomics Enabled by Real-Time Chromatographic Alignment
Targeted mass spectrometry methods produce high quality quantitative data in terms of limits of detection and dynamic range, at the cost of a substantial compromise in throughput compared to methods such as data independent and data de-pendent acquisition. The logistical and experimental issues inhe...
ORGANISM(S): Homo Sapiens 
2021-01-24 | PXD018675 | panorama
ProteomicsDynamic Data Independent Acquisition Mass Spectrometry with Real-Time Retrospective Alignment
We report a data independent acquisition (DIA) strategy that dynamically adjusts the tandem mass spectrometry (MS/MS) windows during the chromatographic separation. The method focuses MS/MS acquisition on the most relevant mass range at each point in time – improving the quantitative sensitivity by ...
ORGANISM(S): Homo Sapiens 
2023-09-18 | PXD038508 | panorama
ProteomicsLinear Ion Trap Global to Targeted Proteomics: A workflow for targeted proteomics assay development using a versatile linear ion trap
Advances in proteomics and mass spectrometry have enabled the study of limited cell populations, such as single-cell proteomics, where high-mass accuracy instruments are typically required. While triple quadrupoles offer fast and sensitive nominal resolution measurements, these instruments are effec...
ORGANISM(S): Mus Musculus 
2025-10-01 | PXD052847 | panorama
ProteomicsDevelopment of highly multiplex targeted proteomics assays in biofluids using a nominal mass ion trap mass spectrometer
The development of targeted assays that monitor biomedically relevant proteins is an important step in bridging discovery experiments to large scale clinical studies. Targeted assays are currently unable to scale to hundreds or thousands of targets. We demonstrate the generation of large-scale assay...
ORGANISM(S): Homo Sapiens Gallus Gallus 
2026-01-12 | PXD065471 | panorama
ProteomicsEvaluating the Performance of the Astral Mass Analyzer for Quantitative Proteomics Using Data Independent Acquisition
We evaluate the quantitative performance of the newly released Asymmetric Track Lossless (Astral) analyzer. Using data independent acquisition, the Orbitrap Astral mass spectrometer quantifies 5 times more peptides per unit time than state-of-the-art Orbitrap mass spectrometers, which have long been...
ORGANISM(S): Homo Sapiens 
2023-09-18 | PXD042704 | panorama
ProteomicsEvaluation of a modified Orbitrap Astral Zoom prototype for quantitative proteomics - Beyond identification lists
Mass spectrometry instrumentation continues to evolve rapidly, yet quantifying these advances beyond conventional peptide and protein detections remains challenging. Here, we evaluate a modified Orbitrap Astral Zoom mass spectrometer (MS) prototype and compare its performance to the standard Orbitra...
ORGANISM(S): Homo Sapiens Gallus Gallus 
2025-10-22 | PXD064536 | panorama
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