Sort   by:  
 Page size 
TranscriptomicsRibosome profiling data obtained from HEK293T cells 30 minutes after treatment with arsenite to a final concentration of 40 M-BM-5M
Eukaryotic cells rapidly reduce protein synthesis in response to various stress conditions. This can be achieved by the phosphorylation-mediated inactivation of a key translation initiation factor, eIF2. However, the persistent translation of certain mRNAs is required for deployment of an adequate s...
ORGANISM(S): Homo sapiens 
2015-01-27 | E-GEOD-55195 | biostudies-arrayexpress
ProteomicsFast and highly efficient affinity enrichment of Azide-A-DSBSO cross-linked peptides.
Cross-linking mass spectrometry is an increasingly used, powerful technique to study protein-protein interactions or to provide structural information. Due to sub-stochiometric reaction efficiencies, cross-linked peptides are usually low abundant. This results in challenging data evaluation and the ...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2020-05-11 | PXD016963 | Pride
Click reaction Affinity enrichment Cross-linking Mass spectrometry Dbco Dsbso 
TranscriptomicsRNA-Seq of A375 cells treated with tetracycline-based crosslinking probes, with and without competition by parent tetracyclines, to show specific binding of the tetracycline analogs Col-3 and doxycycline on rRNA substructures of the human ribosome
Upon finding that the tetracyclines COL-3 and doxycycline target unique rRNA substructures of the 80S ribosome (E-MTAB-7147), we confirmed these putative ribosomal binding sites by showing that tetracycline-based crosslinking probes are specifically competed from these rRNA structures by their paren...
ORGANISM(S): Homo sapiens 
2018-08-31 | E-MTAB-7161 | biostudies-arrayexpress
TranscriptomicsDksA Guards Elongating RNA Polymerase Against Ribosome-Stalling-Induced Arrest
In bacteria, translation-transcription coupling inhibits RNA polymerase (RNAP) stalling. We present evidence suggesting that, upon amino acid starvation, inactive ribosomes promote rather than inhibit RNAP stalling. We developed an algorithm to evaluate genome-wide polymerase progression independent...
ORGANISM(S): Escherichia coli 
2014-04-21 | E-GEOD-54669 | biostudies-arrayexpress
TranscriptomicsRibosome sequencing of the effect of control lentivirus and AARS2 overexpression lentivirus on neonatal rat cardiomyocyte translation
Ribo-seq was performed by Novogene. Briefly, use RNase I to digest the unprotected RNA, leaving only the ribosome-protected mRNA fragments, and sequencing libraries were constructed and carried out on an Illumina Novaseq 6000 SE50.
ORGANISM(S): Rattus norvegicus 
2024-03-01 | E-MTAB-13767 | biostudies-arrayexpress
TranscriptomicsNext Generation Sequencing Facilitates Quantitative Analysis of Wild Type and rea1 mutant total and polysome-bound Transcriptomes
Analysis of gene expression level. The hypothesis tested in the present study was that rea1 mutant influence the translation level of translation and nucleosome assembly associated genes. Results provide important information of the gene translation level regulation of ribosome proteins, elongation...
ORGANISM(S): Zea mays 
2015-04-25 | E-GEOD-67083 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Arabidopsis roots and shoots in response to hypoxia using immunopurified mRNA-ribosome complexes - SuperSeries
This SuperSeries is composed of the following subset Series:; GSE14493: Immunopurified mRNA-ribosome complexes expose cell-type specific plasticity during hypoxia in Arabidopsis root tips; GSE14502: Immunopurified mRNA-ribosome complexes expose cell-type specific plasticity in response to hypoxia in...
ORGANISM(S): Arabidopsis thaliana 
2009-10-20 | E-GEOD-14578 | biostudies-arrayexpress
TranscriptomicsUltra-deep sequencing of ribosome-associated poly-adenylated RNA in early Drosophila embryo reveals hundreds of conserved translating sORFs
Identification of translating small open reading frames (sORFs) through deep sequecing of ribosome-associated poly-adenylated RNA and conservation analysis in early Drosophila embryo
ORGANISM(S): Drosophila melanogaster 
2017-03-05 | E-MTAB-4571 | biostudies-arrayexpress
TranscriptomicsGenome-wide analysis of Musashi-2 targets reveals novel functions in governing epithelial cell migration
The Musashi-2 (Msi2) RNA-binding protein maintains stem cell self-renewal and promotes oncogenesis by enhancing cell proliferation in hematopoietic and gastrointestinal tissues. However, it is unclear how Msi2 recognizes and regulates mRNA targets in vivo and whether Msi2 primarily controls cell gro...
ORGANISM(S): Mus musculus 
2016-04-01 | E-GEOD-71333 | biostudies-arrayexpress
TranscriptomicsThe Small Molecule ISRIB Reverses the Effects of eIF2α Phosphorylation on Translation and Stress Granule Assembly
We recently identified ISRIB as a potent inhibitor of the integrated stress response (ISR). ISRIB renders cells resistant to the effects of eIF2α phosphorylation and enhances long-term memory in rodents (10.7554/eLife.00498). Here we show by genome-wide in vivo ribosome profiling that translation o...
ORGANISM(S): Homo sapiens 
2015-02-27 | E-GEOD-65778 | biostudies-arrayexpress
Sort   by:  
 Page size