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ProteomicsFast and highly efficient affinity enrichment of Azide-A-DSBSO cross-linked peptides.
Cross-linking mass spectrometry is an increasingly used, powerful technique to study protein-protein interactions or to provide structural information. Due to sub-stochiometric reaction efficiencies, cross-linked peptides are usually low abundant. This results in challenging data evaluation and the ...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2020-05-11 | PXD016963 | Pride
Click reaction Affinity enrichment Cross-linking Mass spectrometry Dbco Dsbso 
ProteomicsDSBSO-based XL-MS Analysis of Breast Cancer PDX Tissues to Delineate Protein Interaction Network in Clinical Samples
We applied our previously established Alkyne-A-DSBSO-based in vivo XL-MS analytical platform on two types of breast cancer PDX samples cross-linking. Coupled with the LC-MSn strategy, the experiments enable us to obtain information on cancer-related protein-protein interactions since experimenting o...
ORGANISM(S): Homo sapiens (Human) 
2024-03-20 | PXD046966 | Pride
Breast cancer Pdx Cross-linking mass spectrometry 
ProteomicsIn vivo crosslinking and effective 2D enrichment for interactome studies of the nucleosome.
Cross-linking mass spectrometry has evolved as a powerful technique to study protein-protein interactions and to provide structural information over the past decades. Low reaction efficiencies, and complex matrices lead to challenging system wide crosslink analysis. In this study, we improved and st...
ORGANISM(S): Homo sapiens (Human) 
2025-08-21 | PXD061173 | Pride
Affinity enrichment Ddx39b Xl-ms Dsbso 
ProteomicsA high-efficiency Azide-A-DSBSO cross-linking-MS protocol for identifying thousands of in vivo protein interactions in single day
Enrichable and mass spectrometry (MS)-cleavable crosslinkers have gained popularity in crosslinking-MS (XL-MS) in the past decade. The azide-tagged, acid-cleavable disuccinimidyl bis-sulfoxide (DSBSO) crosslinker offers both these advantages, enabling deep XL-MS interactomics studies in intact cells...
ORGANISM(S): Homo sapiens (Human) Bacillus subtilis 
2025-11-27 | PXD065905 | Pride
High-thoughput Cross-linking mass spectrometry Dsbso 
UnknownOptimized In-Solution and Gas-Phase Chemistry Enables High-Efficiency Interactome Mapping by DSBSO-Based Cross-Linking Mass Spectrometry.
Not available
S-EPMC12828450 | biostudies-literature
ProteomicsA deep XL-proteomic dataset of intact HEK293T cells using DSBSO
Cross-linking mass spectrometry is an emerging method to study protein-protein interactions (PPIs) in intact biological systems. Here, we optimized a workflow for gnerating high coverage maps of PPIs using the enrichable cross-linker Azide-A-DSBSO. This created one of the largest to-date XL-MS datas...
ORGANISM(S): Homo sapiens (Human) 
2024-10-17 | PXD043531 | Pride
Human Deep xl-ms Cell culture 
UnknownFast and Highly Efficient Affinity Enrichment of Azide-A-DSBSO Cross-Linked Peptides.
Not available
S-EPMC7199212 | biostudies-literature
UnknownDSBSO-Based XL-MS Analysis of Breast Cancer PDX Tissues to Delineate Protein Interaction Network in Clinical Samples.
Not available
S-EPMC11296914 | biostudies-literature
ProteomicsA deep XL-proteomic dataset of intact HEK293T cells using DSBSO
Cross-linking mass spectrometry is an emerging method to study protein-protein interactions (PPIs) in intact biological systems. Here, we optimized a workflow for gnerating high coverage maps of PPIs using the enrichable cross-linker Azide-A-DSBSO. This created one of the largest to-date XL-MS datas...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-01-08 | MSV000096805 | MassIVE
ProteomicsCross-link assisted spatial proteomics to map sub-organelle proteomes and membrane protein topology
The specific functions of cellular organelles and sub-compartments depend on their protein content, which can be characterized by spatial proteomics approaches. However, many spatial proteomics methods are limited in their ability to resolve organellar sub-compartments, profile multiple sub-compartm...
ORGANISM(S): Homo sapiens (Human) 
2024-02-21 | PXD046382 | Pride
Synaptic vesicles Xl-ms Mitochondria Spatial proteomics Dsbso 
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