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HEK293 FlpIn T-Rex cells were maintained in Dulbeccos Modified Eagle Medium (DMEM) with 10% fetal bovine serum (FBS), supplemented with 3 g/ml blasticidine and 50 g/ml zeocin. For the siRNA-induced knockdown of TDP-43, 20 nM of TDP-43 stealth siRNA was mixed with 10 L of RNAiMAX following the manufa...
ORGANISM(S): Homo sapiens 
Illumina TRUseq method to generate highly strand-specific next-generation sequencing (NGS) libraries
ORGANISM(S): Mus musculus 
QuantSeq-Rev method to generate highly strand-specific next-generation sequencing (NGS) libraries enabling transcript quantification and identification of the 3'end of polyadenylated RNAs
ORGANISM(S): Mus musculus 
QuantSeq-Rev method to generate highly strand-specific next-generation sequencing (NGS) libraries enabling transcript quantification and identification of the 3'end of polyadenylated RNAs
ORGANISM(S): Homo sapiens 
Illumina TRUseq method to generate highly strand-specific next-generation sequencing (NGS) libraries
ORGANISM(S): Homo sapiens 
Nascent RNAseq in conjunction with Illumina TRUseq method to sequence total RNAs including short lived RNAs using highly strand-specific next-generation sequencing (NGS) libraries
ORGANISM(S): Homo sapiens 
Transcriptional profiling of D. discoideum revealed sets of genes whose expression is enriched in amoebae interacting with different species of bacteria, including sets that appear specific to amoebae interacting with Gram(+), or with Gram(-) bacteria. In a genetic screen utilizing the growth of mut...
ORGANISM(S): Dictyostelium discoideum 
merMEDEA (MEA) is a component of the Polycomb Repressive complex 2 (PRC2) of Arabidopsis thaliana, and a maternal imprinted gene that functions during central cell, embryo and endosperm development. Seeds that develop from mea mutant egg cells abort regardless of the paternal genotype. With this app...
ORGANISM(S): Arabidopsis thaliana 
Cross species comparison of genomes and transcriptomes is a powerful tool for understanding underlying biological processes, accurate functional classification of genes, and discovery of novel elements. The shift to next-generation sequencing platforms has tremendously accelerated the analysis of ge...
ORGANISM(S): Dictyostelium purpureum 
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