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GenomicsE2F7 represses a transcriptional network of oscillating cell cycle genes to control S-phase progression.
E2F transcription factors are known to be important for timely activation of G1/S and G2/M genes required for cell cycle progression, but transcriptional mechanisms for deactivation of cell cycle regulated genes are unknown. Here, we show that E2F7 is highly expressed during mid to late S-phase, occ...
ORGANISM(S): Homo sapiens 
2012-01-04 | E-GEOD-32673 | biostudies-arrayexpress
TranscriptomicsssDNA in S phase compared to G1 phase at different timepoints
To identify origins of replication in Lachancea waltii by mapping regions of ssDNA that are enriched in S phase. L. waltii cells are grown in the presence of hydroxyurea, which slows replication forks and causes the accumulation of ssDNA at the replication fork.
ORGANISM(S): Lachancea waltii 
2012-05-09 | E-GEOD-35253 | biostudies-arrayexpress
TranscriptomicsTranscriptional profiling of MLL5 Knock down vs. control C2C12 myoblasts (S phase enriched)
Knockdown of MLL5 led to deregulation of S phase. To understand the molecular basis for this phenotype, we performed microarray analysis of S phase synchronized myoblasts. Genes differentially regulated by MLL5 knock down were revealed by microarray analysis using NIA15K mouse chips. Control and kno...
ORGANISM(S): Mus musculus 
2010-05-22 | E-GEOD-14931 | biostudies-arrayexpress
TranscriptomicsS phase and HU profiles in wild-type and mutant cells
Total S phase was measured for wild-type cells undergoing meiS and mitS. Early replication origins were mapped in mitS in wild-type cells, and in meiS for wild-type, sml1 delete, rec8 delete and spo11 delete cells. Comparative genome hybridization was used to measure the kinetics and final extent of...
ORGANISM(S): Saccharomyces cerevisiae 
2012-06-18 | E-GEOD-35662 | biostudies-arrayexpress
GenomicsTranscription factor binding in human cells occurs in dense clusters formed around cohesin anchor sites [S-phase/M-phase synchronized cells]
During cell division, transcription factors (TFs) are removed from chromatin twice, during DNA synthesis, and during condensation of chromosomes. How TFs can efficiently find their sites following these stages has been unclear. Here, we have analyzed the binding pattern of expressed TFs in human col...
ORGANISM(S): Homo sapiens 
2013-10-01 | E-GEOD-51290 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of S. pombe reveals Cyclin dependent kinase inhibits re-initiation of a normal S-phase program during G2
To achieve faithful replication of the genome once in each cell cycle, re-initiation of S-phase is prevented in G2 and origins are restricted from re-firing within S-phase. We have investigated the block to re-replication during G2 in fission yeast. The DNA synthesis that occurs when G2/M cyclin d...
ORGANISM(S): Schizosaccharomyces pombe 
2009-05-13 | E-MTAB-105 | biostudies-arrayexpress
TranscriptomicsS-phase time-course of Lachancea kluyveri cells
Haploid cells of Lachancea kluyveri were arrested in G1 phase using Saccharomices cerevisiae alpha factor. After, release in a new media, cells go synchronously through S-phase. One sample is taken every five minutes. Microarrays are used to monitor the change of DNA copy number from 1 to 2, all alo...
ORGANISM(S): Lachancea kluyveri 
2013-02-01 | E-GEOD-37244 | biostudies-arrayexpress
TranscriptomicsGlobal analysis of differential polyadenylation of mRNAs during mitotic cell cycle: S phase versus G2/M phase.
We compared the poly(A) tail length status of mRNAs of HeLa cells between two phases of the mitotic cell cycle: S and G2/M phases. Hundreds of mRNAs were found to be regulated by changes in their poly(A) tail length during mitotic cell cycle in a phase specific manner. Many of these differentially p...
ORGANISM(S): Homo sapiens 
2010-05-07 | E-GEOD-15399 | biostudies-arrayexpress
TranscriptomicsINO80-regulated genes in S-phase after MMS treatment
This is transcription analysis of genes regulated by the INO80 chromatin remodeling complex in S-phase of the cell cycle after MMS treatment. Wildtype and ino80 mutant cells were first synchronized at G1 using alpha-factor, the cells were released in to S-phase in the presence of 0.02% MMS. After 45...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-20 | E-GEOD-18555 | biostudies-arrayexpress
GenomicsConservation of replication timing reveals global and local regulation of replication origin activity.
DNA replication initiates from defined locations called replication origins; some origins are highly active whereas others are dormant and rarely used. Origins also differ in their activation time resulting in particular genomic regions replicating at characteristic times and in a defined temporal o...
ORGANISM(S): Saccharomyces paradoxus 
2012-06-19 | E-GEOD-36045 | biostudies-arrayexpress
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