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ProteomicsFormaldehyde fixation helps preserving proteome state during single-cell proteomics sample processing and analysis
While mass spectrometry-based single cell proteomics (SCP) is gaining significant momentum, it is largely limited to a few laboratories worldwide. The ability to send samples to specialized core facilities, collaborators, would greatly benefit non-specialists laboratories or those unable to afford t...
ORGANISM(S): Homo sapiens (Human) 
2025-02-05 | PXD054445 | Pride
Single-cell proteomics Formaldehyde fixation 
ProteomicsDeep single-cell proteomics quantifies low-abundant embryonic transcription factors and lineage markers during induced pluripotent stem cell differentiation
Single-cell proteomics (SCP) promises to revolutionize biomedicine by providing an unparalleled view of the proteome in individual cells. Here, we present a high-sensitivity SCP workflow identifying >5000 proteins in individual HeLa cells. It also facilitated direct detection of post-translational m...
ORGANISM(S): Homo sapiens (Human) 
2024-08-17 | PXD049211 | Pride
Single-cell proteomics Proteochip evo 96 Orbitrap astral 
ProteomicsDeep single-cell proteomics quantifies low-abundant embryonic transcription factors and lineage markers during induced pluripotent stem cell differentiation
Single-cell proteomics (SCP) promises to revolutionize biomedicine by providing an unparalleled view of the proteome in individual cells. Here, we present a high-sensitivity SCP workflow identifying >5000 proteins in individual HeLa cells. It also facilitated direct detection of post-translational m...
ORGANISM(S): Homo sapiens (Human) 
2024-08-17 | PXD049181 | Pride
Single-cell proteomics Proteochip evo 96 Orbitrap astral 
ProteomicsComprehensive chemical proteomics analyses reveal that the new TRi-1 and TRi-2 compounds are more specific thioredoxin reductase 1 inhibitors than Auranofin
Anticancer drugs that target cellular antioxidant systems have recently attracted much attention. Auranofin (AF) is currently evaluated in several clinical trials as an anticancer agent and targets the cytosolic and mitochondrial forms of the selenoprotein thioredoxin reductase, Txnrd1 and Txnrd2. R...
ORGANISM(S): Mus musculus (Mouse) 
2021-12-15 | PXD028398 | Pride
Inhibition Thioredoxin reductase Cancer Proteomics Mouse 
ProteomicsChemogenomic screening in a patient-derived 3D model of metabolic dysfunction-associated steatohepatitis reveals the CHRM1-TRPM8 axis as a novel module for targeted intervention
Metabolic dysfunction-associated steatohepatitis (MASH) is a leading cause of liver disease. A multitude of compounds have been developed in recent years but, due to lack of efficacy or safety concerns, receiving market approval has been challenging and restricted. These failures emphasize the need...
ORGANISM(S): Homo sapiens (Human) 
2024-11-28 | PXD052956 | Pride
Nash  muscarinic receptor  phenotypic assay  target discovery  chemical probes  fibrosis. 
UnknownEzrin, radixin, and moesin are dispensable for macrophage migration and cellular cortex mechanics.
Not available
S-EPMC11535515 | biostudies-literature
ProteomicsPolymorphism in estrogen receptor binding site causes CD2-dependent sex bias in T cell autoimmune diseases
Complex autoimmune diseases are sexually dimorphic. An interplay between predisposing genetics and sex-related factors likely determines the sex discrepancy in the immune response, but conclusive evidence regarding the underlying molecular mechanisms is lacking. Using forward genetics, we positional...
ORGANISM(S): Mus musculus (Mouse) 
2021-07-15 | PXD024126 | Pride
Estrogen receptor Sexual dimorphism Autoimmune diseases 
ProteomicsPolymorphisms of the vitamin D receptor regulate T cells and T cell dependent inflammatory diseases
Complex autoimmune diseases have proven difficult to dissect down to their causative genetic mechanisms. As a result, epidemiological data from different human association studies are often merged to arrive at a working hypothesis. In one of such examples, lack of sun exposure and consequent lower s...
ORGANISM(S): Mus musculus (Mouse) 
2020-09-28 | PXD019876 | Pride
T cell Inflammatory disease Vitamin d3 
ProteomicsThermal stability variation during transtion to pluripotency
To investigate functional differences between pluripotent stem cells and somatic cells, we measured protein thermal stability and expression after transitions between cell types using allogenic cell lines. To this purpose, we reprogrammed hFF into human iPSCs and initiated undirected differentiation...
ORGANISM(S): Homo sapiens (Human) 
2021-10-05 | PXD018453 | Pride
Pluripotent stem cell  protein thermal stability  proteome  ribosome  translation  shwachman-diamond syndrome. 
ProteomicsSBDS-dependent ribosome destabilization maintains pluripotency via regulation of translation
Unique traits of pluripotent stem cells are not fully known. Using thermal proteome profiling, we identified reduced stability of ribosomes in induced pluripotent cells (iPSCs) compared to that in somatic cells. Also, iPSCs exhibited lower protein synthesis rate and lower expression of a ribosome ma...
ORGANISM(S): Homo sapiens (Human) 
2021-10-05 | PXD015874 | Pride
Thermal proteome profiling Ribosome Pluripotent stem cells Translation 
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