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ProteomicsHLA class I MAPPs Assay in 10 mono-allelic cell lines transfected with SaCas9, AsCas12a and CasΦ
MHC-associated peptide proteomics (MAPPs) enables direct identification of intracellular peptides naturally processed and presented by cells, providing experimental evidence for antigen processing and HLA class I presentation. In this dataset an HLA-A/B/C-focused MAPPs workflow was used on 10 mono-a...
ORGANISM(S): Homo sapiens (Human) 
2026-09-14 | PXD073207 | Pride
ProteomicsRational engineering of minimally immunogenic nucleases for gene therapy
Genome editing using CRISPR-Cas systems is a promising avenue for the treatment of genetic diseases. However, cellular and humoral immunogenicity of genome editing tools, which originate from bacteria, complicates their clinical use. Here we report reduced immunogenicity (Red)(i)-variants of two cli...
ORGANISM(S): Homo sapiens (Human) 
2024-11-07 | PXD054579 | Pride
Sacas9 Mapps Ascas12a Gene therapy 
UnknownEditing Citrus Genome via SaCas9/sgRNA System.
Not available
S-EPMC5732962 | biostudies-literature
GenomicsHomo sapiens
SaCas9 amplicon sequencing
PRJNA1088532 | ENA
GenomicsHomo sapiens isolate:HEK293T
SaCas9 Specificity Screen
PRJNA454765 | ENA
UnknownA detailed protocol for expression, purification, and activity determination of recombinant SaCas9.
Not available
S-EPMC8991255 | biostudies-literature
UnknownHigh-fidelity SaCas9 identified by directional screening in human cells.
Not available
S-EPMC7347106 | biostudies-literature
OtherCRISPR-SaCas9-KKH targeting of miR-21 through a single adeno-associated virus vector prolongs survival of GB-bearing mice
Glioblastoma (GB), the most aggressive tumor of the central nervous system (CNS), has poor patient outcomes with limited effective treatments available. MicroRNA-21 (miR-21) is a known oncogene, abundantly expressed in many cancer types. MiR-21 promotes glioblastoma progression, and lack of miR-21 r...
ORGANISM(S): Mus musculus 
2024-11-27 | GSE277282 | GEO
UnknownMachine learning reveals sequence and methylation determinants of SaCas9-PAM interactions in bacteria.
Not available
S-EPMC12805903 | biostudies-literature
UnknownFull-Length Model of SaCas9-sgRNA-DNA Complex in Cleavage State.
Not available
S-EPMC9867433 | biostudies-literature
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