In vitro experiment of stimulation of monocyte-derived dendritic cells with Saccaromyces cerevisiae in exponential growth phase. This experiment was performed to verify the comparability of microarray experiments using a pathway based logic (Beltrame et al., PLoS 2009) by comparing the analysis of t...
In vitro experiment of stimulation of monocyte-derived dendritic cells with Saccaromyces cerevisiae in exponential growth phase. This experiment was performed to verify the comparability of microarray experiments using a pathway based logic (Beltrame et al., PLoS 2009) by comparing the analysis of t...
To ditinguish different immune response and investigate the rule of proper recognition of fungi, a transcriptional analysis on moDCs exposed to a pathogenic and a harmless fungi was performed. Monocyte-derived dendritic cells were treated with Candida albicans, two different forms of Saccaromyces ce...
DNA double-strand breaks (DSBs) initiate meiotic recombination. Past DSB-mapping studies have used rad50S or sae2? mutants, which are defective in break processing, to accumulate DSBs, and report large (= 50 kb) “DSB-hot” regions that are separated by “DSB-cold” domains of similar size. Substantial ...
In order to establish the optimal conditions to study expression of genes of S. cerevisiae upon interaction with dendritic cells, Saccaromyces cerevisiae cells were cultured in presence of dendritic cells for 4 hours. Dendritic cells were then treated with zymolase, the yeast cells recovered and...
As part of a larger study involving novel HDAC complex inhibitors, cultures were treated with 0.6% DMSO, 400 μM TSA, or 400 μM E6, then ChIP-Seq was performed via Sin3-(3xHA)
