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TranscriptomicsHITS-CLIP analysis of Yb binding sites in Drosophila ovary cell line
piRNAs direct Piwi to repress transposons to maintain genome integrity in Drosophila ovarian somatic cells. piRNA maturation and association with Piwi occur at perinuclear Yb bodies, the centers of piRNA biogenesis. Here, we show that piRNA intermediates arising from the piRNA cluster flamenco (flam...
ORGANISM(S): Drosophila melanogaster 
2014-06-24 | E-GEOD-54875 | biostudies-arrayexpress
ProteomicsAcetylation of p73 in 293T cell
293T cells ectopically expressing FLAG-tagged p73 were treated with AGK2 and p73 was immunoprecipitated. The precipitants were resuspended in deacetylation buffer (50 mM Tris-HCl pH 8.0, 140 mM NaCl, 2 mM MgCl 2 ) and incubated with recombinant SIRT2 protein (10 U). The reaction was terminated by bo...
ORGANISM(S): Homo Sapiens (human) 
2018-09-10 | PXD010411 | JPOST Repository
ProteomicsProteomic identification of PTMs of MAVS 1-200
To identify PTMs of MAVS, we overexpressed His-Flag-tagged hMAVS 1-200 in 293T cells and cell lysates were analyzed by LC-MS/MS.
ORGANISM(S): Homo Sapiens (human) 
2025-07-23 | PXD061814 | JPOST Repository
ProteomicsProteomic identification of PTMs of MAVS 1-200
To identify PTMs of MAVS, we overexpressed His-Flag-tagged hMAVS 1-200 in 293T cells and cell lysates were analyzed by LC-MS/MS.
ORGANISM(S): Homo Sapiens (human) 
2025-07-23 | PXD058405 | JPOST Repository
ProteomicsProteomic identification of MAVS carboxylation site–associated proteins
To identify proteins that differentially interact with carboxylated and non-carboxylated MAVS, we overexpressed Flag-tagged WT or 4A mutant (D53A, E70A, E80A, and D83A) forms of hMAVS in 293T cells and analyzed the interactors using immunoprecipitation followed by mass spectrometry (MS). Cell lysate...
ORGANISM(S): Homo Sapiens (human) 
2025-07-23 | PXD058403 | JPOST Repository
Proteomicsanti-HA IP-MS of Pdzd8-3×HA knock-in mouse neocortex
Pdzd8-3xHA knock-in mice and control littermates at postnatal 10 days were put to sleep using medetomidine hydrochloride, midazolam and butorphanol. Pups were then put on the ice for 5 minutes and exsanguinated by terminal intracardial perfusion with ice-cold 2% paraformaldehyde in PBS. The neocorte...
ORGANISM(S): Mus Musculus (mouse) 
2025-04-18 | PXD052134 | JPOST Repository
ProteomicsProximity biotinylation of PDZD8-TurboID KI HeLa cells
PDZD8-TurboID knocked-in HeLa cells were plated into a 15 cm dish and cultured two overnight. The cells were treated with Biotin at the final concentration of 50 μM, and incubated for 6 hours. The cells were washed twice with ice-cold Hepes-saline and lysed in 6 M guanidine-HCl containing 100 mM HEP...
ORGANISM(S): Homo Sapiens (human) 
2025-04-18 | PXD052135 | JPOST Repository
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