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ProteomicsIn vitro reconstitution of epigenetic reprogramming in the human germ line
M1-BTAG iPSCs or TET1-KO iPSCs were analyzed by 8% SDS-PAGE, followed by trypsin digestion and LC/MS/MS with an Orbitrap Fusion Lumos linked with an Ultimate 3000 pump and HTC-PAL autosampler. Acquired data files were processed using FragPipe v20.0 (MSFragger v3.8, Philosopher v5.0.0, IonQuant v1.9....
ORGANISM(S): Homo Sapiens (human) 
2024-04-25 | PXD048118 | JPOST Repository
TranscriptomicsTranscription profiling of mouse germ cells with conditional Nanog knockdown.
Single Cell Microarray
E10.5 genital ridges (TM at E7.5) incubated in 0.5mM EDTA/ PBS for 20 minutes at 37ï¾°C were transferred to 2% BSA/ PBS. PGCs were collected from the genital ridges pierced with fine glass needles. Released PGCs were identified by their morphological characteristics and tr...
ORGANISM(S): Mus musculus 
2009-11-16 | E-MEXP-2411 | biostudies-arrayexpress
TranscriptomicsThe effect of HP1-gamma deficiency on primordial germ cells
We compared the gene expression profiles of the HP1 gamma -/- vs wild-type PGCs to examine the molecular mechanisms of HP1g in PGC development. Although HP1g is suggested to be involved in PGC proliferation, our microarray data indicated that HP1g does not directly control PGC cell-cycle progression...
ORGANISM(S): Mus musculus 
2011-07-31 | E-GEOD-27294 | biostudies-arrayexpress
TranscriptomicsReplication-coupled passive DNA demethylation for the erasure of genome imprints in mice
Genome-wide DNA demethylation, including the erasure of genome imprints, in primordial germ cells (PGCs), is critical as a first step for creating the totipotent epigenome in the germ line. Here, we provide evidence that contrary to the prevailing model involving active DNA demethylation, imprint er...
ORGANISM(S): Mus musculus 
2013-03-04 | E-GEOD-40412 | biostudies-arrayexpress
TranscriptomicsA developmental coordinate of the spectrum of pluripotency among mice, monkeys, and humans
The epiblast (EPI) is the origin of all somatic and germ cells in mammals, and of the spectrum of pluripotent stem cells (PSCs) in vitro. To explore the ontogeny of human/primate pluripotency, we performed comprehensive single-cell RNA sequencing for pre- and post-implantation EPI development in cyn...
ORGANISM(S): Mus musculus 
2016-08-24 | E-GEOD-74767 | biostudies-arrayexpress
GenomicsPRDM14 Ensures Naïve Pluripotency through Dual Regulation of Signaling and Epigenetic Pathways in Mouse Embryonic Stem Cells
Mouse embryonic stem cells (mESCs) fluctuate between a naïve inner cell mass (ICM)-like state and a primed epiblast-like state of pluripotency in serum, but are harnessed exclusively in a distinctive, naïve state of pluripotency that more faithfully captures the ICM state (the ground state) with i...
ORGANISM(S): Mus musculus 
2013-01-18 | E-GEOD-42616 | biostudies-arrayexpress
TranscriptomicsCell-to-cell gene expression variability followed by reinforcement progressively segregates lineages in the early mouse embryo
Mammalian embryonic development begins with the specification and segregation of the two extra-embryonic lineages, trophectoderm and primitive endoderm, from the pluripotent embryonic lineage, the epiblast. To establish a map of epiblast (EPI) versus primitive endoderm (PrE) lineage segregation whic...
ORGANISM(S): Mus musculus 
2013-11-26 | E-MTAB-1681 | biostudies-arrayexpress
TranscriptomicsInduction of the mouse germ cell fate by transcription factors in vitro [exp2]
The germ cell lineage ensures the continuity of life through the generation of male and female gametes, which unite to form a totipotent zygote. We have established a culture system that recapitulates the mouse germ-cell specification pathway: Using cytokines, embryonic stem cells (ESCs)/induced plu...
ORGANISM(S): Mus musculus 
2013-08-04 | E-GEOD-46854 | biostudies-arrayexpress
TranscriptomicsInduction of the mouse germ cell fate by transcription factors in vitro [exp1]
The germ cell lineage ensures the continuity of life through the generation of male and female gametes, which unite to form a totipotent zygote. We have established a culture system that recapitulates the mouse germ-cell specification pathway: Using cytokines, embryonic stem cells (ESCs)/induced plu...
ORGANISM(S): Mus musculus 
2013-08-04 | E-GEOD-43775 | biostudies-arrayexpress
TranscriptomicsPRDM14 Ensures a Naïve Pluripotency by a Dual Mechanism Involving Signaling and Epigenetic Pathways in Mouse Embryonic Stem Cells
Mouse embryonic stem cells (mESCs) fluctuate between a naïve inner cell mass (ICM)-like state and a primed epiblast-like state of pluripotency in serum, but are harnessed exclusively in a distinctive, apparently more naïve state of pluripotency (the ground state) with inhibitors for mitogen-activate...
ORGANISM(S): Mus musculus 
2013-01-18 | E-GEOD-42580 | biostudies-arrayexpress
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