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Mesenchymal stem cells (MSCs) And osteolineage cells contribute to the hematopoietic stem cell (HSC) Niche in the bone marrow of long bones. However, Their developmental relationships remain unclear. Here we demonstrate that different MSC populations in the developing marrow of long bones have disti...
ORGANISM(S): Mus musculus 
Global restriction of protein synthesis is a hallmark of cellular stress. Using fusel alchohol addition, we monitor the transcript level and also the translation status for each RNA using cycloheximide to freeze elongating ribosomes. Polyribosome fractionation of cell extracts was used to seperate h...
ORGANISM(S): Saccharomyces cerevisiae 
Global restriction of protein synthesis is a hallmark of cellular stress. Using amino-acid starvation, we monitor the transcript level and also the translation status for each RNA using cycloheximide to freeze elongating ribosomes. Polyribosome fractionation of cell extracts was used to seperate hig...
ORGANISM(S): Saccharomyces cerevisiae 
Microarray analysis of exosomal miRNAs vs the miRNAs of their respective donor cells. To determine the miRNA repertoires of exosomes secreted by immune cells, we isolated exosomes from cell supernatants of the Raji B cell line, the Jurkat-derived J77 T cell line, and primary dendritic cells (DCs) de...
ORGANISM(S): Homo sapiens 
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