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ProteomicsPolysome profiling reveals translational control of gene expression in the human malaria parasite Plasmodium falciparum
MudPIT analysis of Plasmodium polysomes. TCA precipitated pellet from Plasmodium falciparum polysomes (~50ug) was solubilized in TRIS-HCl pH8.5 and Urea; reduced and alkylated. The protein suspension was digested overnight using Endoproteinase Lys-C followed by a second overnight digestion at 37°C ...
ORGANISM(S): Plasmodium falciparum 
2013-10-31 | PXD000553 | Pride
Transcription Cell cycle Malaria Translation 
ProteomicsSpop restrains inflammation-induced emergency hematopoiesis by targeting MyD88
Innate immune cells, and myeloid lineage cells in particular, constitute the first line ofdefense for the containment of invading pathogens. To meet the augmented demand of myeloidcells in response to systemic infections, inflammatory signals trigger a rapid transcriptional response inhematopoietic ...
ORGANISM(S): Homo sapiens (Human) 
2019-07-12 | PXD009469 | Pride
Spop 
ProteomicsMudPIT analyses of the proteins co-purified with wild type-Hzg-3XHA/Flag and phosphatase dead-Hzg-3XHA/Flag affinity purifications from Drosophila melanogaster S2 cells
Protein Complexes Purification- Stable S2 cell expressing Hzg albeit low level, lines expressing C-terminally 3XHA-Flag-tagged wild-type and phosphatase dead (PD) Hzg were generated. A parental cell line was used as a negative control. Nuclear and cytoplasmic extracts from the Hzg-WT, Hzg-mutant and...
ORGANISM(S): Drosophila Melanogaster (ncbitaxon:7227) 
2019-01-08 | MSV000083305 | MassIVE
ProteomicsMudit analyses of the proteins substrates candidate of NRBP1 using TR-TUBE system
293 stable cell line expressing NRBP1 or NRBP1 mutant were transfected with HA-TR-TUBE using TR-TUBE system to protect the degradation of the ubiquitin chains of the potential ligase substrate. The complexes were pulled down using anti-HA antibody and subsequently digested with modified Trypsin. Ant...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-10-04 | MSV000084419 | MassIVE
ProteomicsMudPIT analysis of the mRNA interactome from Plasmodium falciparum
The Plasmodium falciparum strain 3D7 was cultured in human O+ erythrocytes at 5% haematocrit as previously described (Trager and Jensen, 1976). Cultures were synchronized twice at ring stage with 5% D-sorbitol treatments performed eight hours apart (Lambros and Vanderberg, 1979). Cultures (8% parasi...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Plasmodium Falciparum 3d7 (ncbitaxon:36329) 
2016-03-29 | MSV000079612 | MassIVE
ProteomicsMudPIT analysis of proteins interacting with the retinoic acid coactivator Rere/Atrophin2 in mouse embryos
To identify proteins binding to Rere, we first generated a mouse colony homozygous for both the T-Cre transgene and the RS-Rere-HA allele. Thus, all the embryos generated by crossing these mice expressed Rere-HA only in mesodermal tissues. We collected around 600 E10.5 embryos and prepared whole-cel...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2017-04-13 | MSV000080920 | MassIVE
ProteomicsMudPIT analyses of the proteins co-purified with Ada2b-PB-FLAG-HA FLAG-affinity purified from Drosophila melanogaster S2 cells
Protein Complexes Purification- Stable S2 cell lines expressing Ada2b (isoform B; NP_001027151.1) in the pRmHa3-CHA2FL2 (Ada2b-PBH2F2) were maintained at 1-2 e6 cells/mL in SFX medium (HyQ SFX-Insect). Cells stably transfected with empty vector served as negative controls. Protein complexes containi...
ORGANISM(S): Drosophila Melanogaster (ncbitaxon:7227) 
2018-11-20 | MSV000083153 | MassIVE
ProteomicsStructure and function of brain derived Orb2 amyloid filaments linked to memory
To isolate the different brain-derived Orb2 species, we used approximately 3 million 3 to 7- day-old adult Drosophila melanogaster Canton-S flies. First, flies were collected in 50 ml tubes and snap-frozen in liquid nitrogen. The heads were separated from the body by vortexing for 5-10 sec, and imme...
ORGANISM(S): Drosophila Melanogaster (ncbitaxon:7227) 
2019-11-13 | MSV000084579 | MassIVE
ProteomicsMudPIT analyses of the proteins associated with FBXW5 in HEK293T cells
Nutrient deprivation of the cell mobilizes an extensive amount of membrane to form and grow the autophagosome, allowing the progression of autophagy. By providing membranes and stimulating LC3 lipidation, Coat Protein Complex II (COPII) promotes autophagosome biogenesis. Here, we show that the F-b...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-12-29 | MSV000083289 | MassIVE
ProteomicsMudPIT analysis of the polysome-associated proteins from Plasmodium falciparum
The Plasmodium falciparum strain 3D7 was cultured in human O+ erythrocytes at 5% haematocrit as previously described (Trager and Jensen, 1976). Cultures were synchronized twice at ring stage with 5% D-sorbitol treatments performed eight hours apart (Lambros and Vanderberg, 1979). Cultures (8% parasi...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Plasmodium Falciparum 3d7 (ncbitaxon:36329) 
2016-03-29 | MSV000079613 | MassIVE
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