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Although immune mechanisms have been described for Peyer’s patch function, in discriminating food nutrients and commensal microflora from pathogenic challenge, an understanding of gene expression patterns associated with normal intestinal homeostasis is lacking. The effects of commensal bacterial co...
ORGANISM(S): Sus scrofa 
Protein Complexes Purification: We generated 293FRT cell lines that stably express FLAG-tagged ICE1 (Interactor of Little Elongation Complex ELL subunit 1; KIAA0947). We extracted nuclear proteins in the presence of Benzonase to digest nucleic acids, including both DNA and RNA, and purified the lit...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-02-20 | MSV000083465 | MassIVE
Fertilized eggs (wild-type, ikke-1, or allo-1 in four, three, or three biological replicates, respectively) were collected from approximately 10,000–20,000 adult hermaphrodites, equivalent to 4–7 plates of 10 cm dish culture, at 60–72 h after the L1 stage using the bleaching method. Eggs were suspen...
ORGANISM(S): Caenorhabditis Elegans 
Roux-en-Y gastric bypass (RYGB) surgery reduces weight in obese patients. A marked decrease in blood glucose levels occurs before weight loss; however, key molecules that improve glycemic profile remain largely unknown. We used the RYGB surgery model in diet-induced obese (DIO) mice to monitor the p...
ORGANISM(S): Mus musculus (Mouse) 
2020-01-23 | PXD015276 | Pride
Fertilized eggs (wild type or ikke-1 mutant) were collected from approximately 10,000 or 70,000 adult hermaphrodites, equivalent to 5 or 35 plates of 10-cm dish culture, at 60–72 h after the L1 stage using the bleaching method, and immediately frozen in liquid nitrogen. The eggs were thawed on ice i...
ORGANISM(S): Caenorhabditis Elegans 
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