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Burkholderia cenocepacia J2315 was grown at 37 degrees centigrade in LB broth to the beginning of stationary phase (18 hours incubation time).
The expression profile was compared to cultures harvested in mid-log phase (7 hours incubation time).
ORGANISM(S): Burkholderia cenocepacia 
B. cenocepacia J2315 was grown on LB medium to mid-stationary phase at O.D. 0.5 at 150 rpm in a shaking incubator at two different temperatures: 37 degrees and 20 degrees centigrade.
ORGANISM(S): Burkholderia cenocepacia 
B. cenocepacia J2315 was grown to mid-stationary phase in basal salt medium with two different substrates: 20 mM glucose or 40 mM glycerol. Cells were harvested after 30 hours incubation at 37 degrees centigrade.
The expression profile was compared to cells grown on the same medium and harvested...
ORGANISM(S): Burkholderia cenocepacia 
B. cenocepacia J2315 was grown to mid-log phase in different media: LB broth, iso-sensitest broth, basal salt medium with glucose
at pH 7 and pH 6, basal salt medium at pH 7 with a reduced iron content, and basal salt medium with glycerol
ORGANISM(S): Burkholderia cenocepacia 
B. cenocepacia J2315 was exposed to heat stress and to stress form reactive oxygen species.
To expose the cultures to heat stress, cells were grown at 37ºC to an O.D. of 0.4 to 0.45 and then transferred into a different shaking incubator at 42.5ºC, incubated for 1 hour at 150 rpm and harvested.<...
ORGANISM(S): Burkholderia cenocepacia 
B. cenocepacia J2315 was grown on LB medium to mid-stationary phase at OD 0.3 under full aeration and then transferred into a 50 ml centrifuge tube and placed upright into a CampyGen Compact (Oxoid, Basingstoke, UK) plastic pouch containing the gas generating paper sachet. The pouch was sealed immed...
ORGANISM(S): Burkholderia cenocepacia 
Burkholderia cenocepacia J2315 was grown in LB broth to the beginning of stationary phase. The expression profile was compared to cultures harvested in mid-log phase.
ORGANISM(S): Burkholderia cenocepacia 
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