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TranscriptomicsmiRNA expression in aging skin
Fibroblasts were isolated from healthy skin, donors were young, middlege and old. We used Affymetrix miRNA microarrays to detail the global expression of miRNAs. Primary fibroblasts were isolated from healthy skin, donnors were young, middlege and old and kept in culture for a short time in order to...
ORGANISM(S): Homo sapiens 
2014-02-25 | E-GEOD-55299 | biostudies-arrayexpress
TranscriptomicsQuantification of regenerative potential in primary human mammary epithelial cells
We present an organoid regeneration assay in which freshly dissociated human mammary epithelial cells from healthy donors are grown in adherent/rigid or floating/compliant collagen I gels. In both conditions, luminal progenitors (CD49f+EpCAM+) form spheres, whereas basal cells (CD49fhiEpCAM-) genera...
ORGANISM(S): Homo sapiens 
2015-07-02 | E-GEOD-64248 | biostudies-arrayexpress
TranscriptomicsGene expression profiling of skeletal myoblast differentiation and exposure to TNF-alpha or IGF1
Skeletal myocyte differentiation involves fusion of myoblasts to multinucleated myotubes. In vitro differentiation can be induced by serum withdrawal. The differentiation process is negatively regulated by pathological concentrations of TNF-alpha but can be positively regulated by IGF1. This study f...
ORGANISM(S): Mus musculus 
2015-04-05 | E-MTAB-3474 | biostudies-arrayexpress
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