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To analyze proteins copurified with GFP-ALLO-1, worm eggs expressing GFP-ALLO-1 or GFP in the atg-11 background were lysed in co-IP buffer, and GFP-ALLO-1 or GFP was immunoprecipitated with GFP-Trap-agarose beads. Proteins on the beads were directly digested with Trypsin overnight. Supernatants were...
ORGANISM(S): Caenorhabditis Elegans 
To analyze phosphorylation sites of ALLO-1, worm eggs expressing GFP-ALLO-1 in the atg-11 or ikke-1 background were lysed in co-IP buffer, and GFP-ALLO-1 was immunoprecipitated with GFP-Trap-agarose beads. Proteins on the beads were directly digested with Trypsin overnight. Supernatants were collect...
ORGANISM(S): Caenorhabditis Elegans 
To analyze phosphorylation sites of ALLO-1, worm eggs expressing GFP-ALLO-1 in the atg-11 or ikke-1 background were lysed in co-IP buffer, and GFP-ALLO-1 was immunoprecipitated with GFP-Trap-agarose beads. Proteins on the beads were directly digested with Trypsin overnight. Supernatants were collect...
ORGANISM(S): Caenorhabditis Elegans 
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