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UnknownPronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.
Not available
S-EPMC3001095 | biostudies-literature
UnknownIntravenous Delivery of piggyBac Transposons as a Useful Tool for Liver-Specific Gene-Switching.
Not available
S-EPMC6274756 | biostudies-literature
TranscriptomicsDownregulation of the Wnt antagonist Dkk2 links loss of Sept4 and myofibroblastic transformation of hepatic stellate cells
Background/Aims: Sept4, a subunit of the septin cytoskeleton specifically expressed in quiescent hepatic stellate cells (HSCs), is downregulated through transdifferentiation to fibrogenic and contractile myofibroblastic cells. Since Sept4?/? mice are prone to liver fibrosis, we examined the unknown ...
ORGANISM(S): Mus musculus 
2012-06-02 | E-GEOD-24588 | biostudies-arrayexpress
Unknowni-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases.
Not available
S-EPMC5828090 | biostudies-literature
UnknownEfficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System.
Not available
S-EPMC5751213 | biostudies-literature
UnknownDirect Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations.
Not available
S-EPMC4581225 | biostudies-literature
TranscriptomicsHeLa cells consist of two cell types, as evidenced by cytochemical staining for alkaline phosphatase activity: A possible model for cancer stem cell study
Alkaline phosphatase (ALP) is known to be a marker for several somatic stem cells and cancer cells. We found that human squamous cell carcinoma HeLa cells are comprised by ALP-positive and negative cells. Single cell-derived colony assay revealed that the former cells are labile with respect to ALP ...
ORGANISM(S): Homo sapiens 
2013-04-23 | E-GEOD-42222 | biostudies-arrayexpress
UnknownThe piggyBac-Based Gene Delivery System Can Confer Successful Production of Cloned Porcine Blastocysts with Multigene Constructs.
Not available
S-EPMC5037703 | biostudies-literature
TranscriptomicsDevelopment of miRNA expression signatures of MEFs deficient in a ER stress mediator ATF6a for tunicamycin treatment
To further development of our miRNA expression approach to ER stress, we have employed miRNA microarray expression profiling as a discovery platform to identify ER stress-responsible ones. Mouse embryonic fibroblasts (MEFs) deficient in a ER stress mediator ATF6a were treated with tunicamycin for ...
ORGANISM(S): Mus musculus 
2015-01-18 | E-GEOD-35176 | biostudies-arrayexpress
TranscriptomicsDevelopment of miRNA expression signatures of MEFs deficient in a ER stress mediator XBP1 for tunicamycin treatment
To further development of our miRNA expression approach to ER stress, we have employed miRNA microarray expression profiling as a discovery platform to identify ER stress-responsible ones. Mouse embryonic fibroblasts (MEFs) deficient in a ER stress mediator XBP1 were treated with tunicamycin for 1...
ORGANISM(S): Mus musculus 
2015-01-18 | E-GEOD-35174 | biostudies-arrayexpress
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