Sort   by:  
 Page size 
MetabolomicsNiacin restriction with NAMPT-inhibition is synthetic lethal to neuroendocrine carcinoma (Cell NAMPTi treatment)

NAMPT plays a major role in NAD biosynthesis in many cancers and is an attractive potential cancer target. However, factors dictating therapeutic efficacy of NAMPT inhibitors (NAMPTi) are unclear. We report that neuroendocrine phenotypes predict lung and prostate carcinoma vulnerability to NAMPTi...

2023-10-10 | MTBLS7251 | MetaboLights
MetabolomicsNiacin restriction with NAMPT-inhibition is synthetic lethal to neuroendocrine carcinoma (Mouse NAMPT targeted therapy)

NAMPT plays a major role in NAD biosynthesis in many cancers and is an attractive potential cancer target. However, factors dictating therapeutic efficacy of NAMPT inhibitors (NAMPTi) are unclear. We report that neuroendocrine phenotypes predict lung and prostate carcinoma vulnerability to NAMPTi...

2023-10-10 | MTBLS7252 | MetaboLights
MetabolomicsNiacin restriction with NAMPT-inhibition is synthetic lethal to neuroendocrine carcinoma (Mouse NAD targeted time-course)

NAMPT plays a major role in NAD biosynthesis in many cancers and is an attractive potential cancer target. However, factors dictating therapeutic efficacy of NAMPT inhibitors (NAMPTi) are unclear. We report that neuroendocrine phenotypes predict lung and prostate carcinoma vulnerability to NAMPTi...

2023-10-10 | MTBLS7253 | MetaboLights
ProteomicsTMT-based phosphoproteomics of WT, ikke-1, and allo-1 eggs
Fertilized eggs (wild-type, ikke-1, or allo-1 in four, three, or three biological replicates, respectively) were collected from approximately 10,000–20,000 adult hermaphrodites, equivalent to 4–7 plates of 10 cm dish culture, at 60–72 h after the L1 stage using the bleaching method. Eggs were suspen...
ORGANISM(S): Caenorhabditis Elegans 
2024-02-01 | PXD048126 | JPOST Repository
ProteomicsGFP-Trap of EPG-7 to identify phosphorylation sites
Fertilized eggs (wild type or ikke-1 mutant) were collected from approximately 10,000 or 70,000 adult hermaphrodites, equivalent to 5 or 35 plates of 10-cm dish culture, at 60–72 h after the L1 stage using the bleaching method, and immediately frozen in liquid nitrogen. The eggs were thawed on ice i...
ORGANISM(S): Caenorhabditis Elegans 
2024-02-01 | PXD048129 | JPOST Repository
ProteomicsT74-pT74-ALLO1-PRM
To analyze phosphorylation sites of ALLO-1, worm eggs expressing GFP-ALLO-1 in the atg-11 or ikke-1 background were lysed in co-IP buffer, and GFP-ALLO-1 was immunoprecipitated with GFP-Trap-agarose beads. Proteins on the beads were directly digested with Trypsin overnight. Supernatants were collect...
ORGANISM(S): Caenorhabditis Elegans 
2018-01-31 | PXD007849 | JPOST Repository
ProteomicsGFP-Trap-IP-phospho
To analyze phosphorylation sites of ALLO-1, worm eggs expressing GFP-ALLO-1 in the atg-11 or ikke-1 background were lysed in co-IP buffer, and GFP-ALLO-1 was immunoprecipitated with GFP-Trap-agarose beads. Proteins on the beads were directly digested with Trypsin overnight. Supernatants were collect...
ORGANISM(S): Caenorhabditis Elegans 
2018-01-31 | PXD007848 | JPOST Repository
ProteomicsGFP-Trap-IP-MS
To analyze proteins copurified with GFP-ALLO-1, worm eggs expressing GFP-ALLO-1 or GFP in the atg-11 background were lysed in co-IP buffer, and GFP-ALLO-1 or GFP was immunoprecipitated with GFP-Trap-agarose beads. Proteins on the beads were directly digested with Trypsin overnight. Supernatants were...
ORGANISM(S): Caenorhabditis Elegans 
2018-01-31 | PXD007847 | JPOST Repository
Sort   by:  
 Page size