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ProteomicsMultiple roles for PARP1 in ALC1-dependent nucleosome remodeling
XL-MS mass spectrometry analysis Cross-linked peptides were resolved for mass spectrometry analysis using a Dionex UltiMate 3000 RSLnano liquid chromatography system. Peptides were initially loaded from the autosampler onto an Acclaim PepMap 100 C18 LC Trap Cartridge (0.3 mm inside diameter, 5 mm le...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-04-10 | MSV000087174 | MassIVE
ProteomicsMudPIT analyses of the proteins co-purified with the Interactor of Little Elongation Complex ELL subunit 1 (FLAG-ICE1), WT MED26, and D2G8 NTD-mutant MED26, affinity-purified from HEK293T cell lines
Protein Complexes Purification: We generated 293FRT cell lines that stably express FLAG-tagged ICE1 (Interactor of Little Elongation Complex ELL subunit 1; KIAA0947). We extracted nuclear proteins in the presence of Benzonase to digest nucleic acids, including both DNA and RNA, and purified the lit...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-02-20 | MSV000083465 | MassIVE
ProteomicsMudit analyses of the proteins substrates candidate of NRBP1 using TR-TUBE system
293 stable cell line expressing NRBP1 or NRBP1 mutant were transfected with HA-TR-TUBE using TR-TUBE system to protect the degradation of the ubiquitin chains of the potential ligase substrate. The complexes were pulled down using anti-HA antibody and subsequently digested with modified Trypsin. Ant...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-10-04 | MSV000084419 | MassIVE
ProteomicsProteome analysis of brain vessels (the anterior cerebral artery and the middle cerebral artery) from 24-month-old Htra1 KO and wild type mice
CARASIL (cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy) is a hereditary cerebral small-vessel disease caused by loss of function mutations of HTRA1, which is a serine protease with a variety of targets, including extracellular matrix proteins. We isolate...
ORGANISM(S): Mus Musculus (mouse) 
2021-11-02 | PXD022298 | JPOST Repository
ProteomicsProteome analysis of brain vessels (the anterior cerebral artery and the middle cerebral artery) from 24-month-old candesartan-treated and nontreated Htra1 KO mice
CARASIL (cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy) is a hereditary cerebral small-vessel disease caused by loss of function mutations of HTRA1, which is a serine protease with a variety of targets, including extracellular matrix proteins. Htra1-KO m...
ORGANISM(S): Mus Musculus (mouse) 
2021-11-16 | PXD023477 | JPOST Repository
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