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ProteomicsIdentification of RNA helicases that mediate processing of tRNAs into distinct tRNA fragments by LC-MS/MS.
Specific environmental insults cause the limited fragmentation of transfer RNAs (tRNAs) into tRNA-derived small RNAs (tsRNAs), which have been implicated in a wide range of biological processes. tRNA fragmentation results from endonucleolytic activities targeting single-stranded tRNA regions. Howeve...
ORGANISM(S): Homo sapiens (Human) 
2023-01-09 | PXD029869 | Pride
Ddx5x Trna fragments Ddx3x Tsrna Rna helicase enzymes 
TranscriptomicsHigh-throughput sequencing of small RNAs (25-70 nt) from Drosophila wild-type and Dnmt2 mutants
Dnmt2 genes are highly conserved tRNA methyltransferases with biological roles in cellular stress responses. The absence of obvious mutant phenotypes under laboratory conditions suggested a function for Dnmt2 under non-physiological conditions. Indeed, Dnmt2 has recently been implicated in various a...
ORGANISM(S): Drosophila melanogaster 
2012-11-12 | E-GEOD-35981 | biostudies-arrayexpress
ProteomicsSpatiotemporal proteomic profiling of Huntington's disease mice reveals widespread loss of protein function
Huntington's disease (HD) is characterized by the aggregation of polyglutamine-expanded huntingtin (HTT), proceeding from soluble oligomers to end-stage inclusions. The molecular mechanisms of how protein aggregation leads to neuronal dysfunction are not well understood. We employed mass spectrometr...
ORGANISM(S): Mus musculus (Mouse) 
2017-11-24 | PXD004973 | Pride
Huntington's disease / inclusion bodies / neurodegeneration / quantitative proteomics 
TranscriptomicsSite-specific cytosine-5 methylation by the mouse RNA methyltransferases Dnmt2 and NSun2 promotes tRNA stability and protein synthesis
Mouse embryonic fibroblast (MEFs) cell lines and liver samples from Dnmt2 and Nsun2 single mutant, double mutant and wildtype mice were used to identify potential mRNA substrates of both proteins. MEFs were derived from day 13.5 embryos and immortalized by transfection with a plasmid expressing the ...
ORGANISM(S): Mus musculus 
2012-07-03 | E-GEOD-36918 | biostudies-arrayexpress
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