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To understand how the NAC transcription factor KIL1 regulates age-induced senescence and cell death in maize silks, we need to get a genome-wide view on its downstream targets. We propose to compare the transcriptome profiles of GOF and LOF transgenic silk tissue with the profile of wild-type B104 s...
ORGANISM(S): Zea mays 
To fully characterize the extent of genomic changes in senescent silk, we performed a time-course tissue harvesting. Material was collected at four time points: 3-, 7-, 11- and 15 days after silk emergence (DASE). Maize inbred line B104; 2 cm of basal part of silk from rings 6-10
ORGANISM(S): Zea mays 
To identify targets of the NAC transcription factor SOMBRERO (SMB, AT1G79580), we used a dexamethasone-inducible p35S::SMB-GR line (DOI: 10.1105/tpc.109.072272), comparing gene expression in dexamethasone-treated and mock-treated 5-day old seedlings 6 hours after estradiol or mock treatment.
ORGANISM(S): Arabidopsis thaliana 
To identify targets of the C2H2 zinc finger protein ZINC 34 FINGER OF ARABIDOPSIS THALIANA 14 (ZAT14, AT5G03510), we used an estradiol-inducible system for inducible expression of ZAT14, comparing gene expression in estradiol-treated plants and mock-treated 5-day old seedlings 8 hours after estradio...
ORGANISM(S): Arabidopsis thaliana 
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