Pure cultures of N. viennensis were subjected to copper limitation using the copper chelator TETA. Two samples that were subjected to copper limiation for approximately two weeks were used for protein extraction and subsequent proteomic analysis.
Information on the files related to the respective experiments can be found in the metadata file titled “Metadata_for_Nviennensis_Experiments.xlsx”. The data found here encompasses three related mass spectrometry experiments: 1.) BN-PAGE: Biomass of Nitrososphaera viennensis grown in a bioreactor w...
Biomass of N. cavascurensis grown in a bioreactor was lysed and membrane fractions were harvested using an ultracentrifuge. Membrane proteins were extracted using n-dodecyl-beta-D-maltoside (DDM) and loaded on a 3-12% gradient blue native PAGE gel. Selected bands were cut out and analyzed via mass...
Proteomic expression analysis of Ca. Lokiarchaeum ossiferum, strain B35, grown using casein hydrolysate as the main carbon source under anaerobic conditions
The ubiquitously distributed ammonia-oxidizing archaea generate energy from ammonia and build cell mass from inorganic carbon sources, thereby contributing to both the global nitrogen and carbon cycles. However, little is known about the regulation of their predicted core carbon metabolism. A the...
The goal of the experiment was to observe metabolic responses on the proteome level to the different stress conditions (including the selective inhibition of obligate syntrophic partners and heat shock) in the Ca. L. ossiferum. The experiment was done is biological triplicates: three different cultu...
Proteomic expression analysis of Ca. Lokiarchaeum ossiferum, strain B36, grown using casein hydrolysate as the main carbon source under anaerobic conditions
This dataset was generated using a crosslinking immunoprecipitation–mass spectrometry (IP–MS) approach to identify proteins interacting with Actin. Cultured cells were mildly fixed with formaldehyde to preserve protein–protein interactions, quenched, and lysed under gentle detergent conditions. Acti...
