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ProteomicsEmergence of enhanced metabolic entanglement during experimental evolution of an interkingdom microbial community
Obligate symbioses have likely evolved through multiple intermediate steps, resulting in a gradual erosion of independence of initially autonomous entities. Here we observed progression towards an increased entanglement for an engineered mutualistic consortium between Escherichia coli and Saccharomy...
ORGANISM(S): Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2024-08-08 | PXD047443 | Pride
S.cerevisiae E.coli 
ProteomicsComparison of different sample preparation protocols reveals lysis buffer specific extraction biases in gram-negative bacteria and human cells
We evaluated different in-solution and FASP-based sample preparation strategies for absolute protein quantification. Label-free quantification (LFQ) was employed to compare different sample preparation strategies in the bacterium Pseudomonas aeruginosa and human embryonic kidney cells (HEK) and note...
ORGANISM(S): Homo sapiens (Human) Pseudomonas aeruginosa PAO1 
2015-10-06 | PXD001511 | Pride
Hek293 Pseudomonas aeruginosa 
ProteomicsRatio accuracy evaluation and correction using tandem mass tags
The multiplexing capabilities of isobaric mass tag based protein quantification, such as Tandem Mass Tags (TMT) or Isobaric Tag for Relative and Absolute Quantitation (iTRAQ) have dramatically increased the scope of Mass Spectrometry (MS) based proteomics studies. Not only does the technology allow ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-08-27 | MSV000082855 | MassIVE
TranscriptomicsRNA-seq of chimpanzee (Pan troglodytes), gorilla (Gorilla gorilla), gibbon (Hylobates lar) and marmoset (Callithrix jacchus) adult testis
RNA sequencing (RNA-seq) was used to annotate chimpanzee, gorilla, gibbon, and marmoset genome and transcript isoforms in adult testis. This dataset is part of a larger evolutionary study of adult testis at the single-nucleus level (97,521 single-nuclei in total) across mammals including 10 represen...
ORGANISM(S): Gorilla gorilla 
2021-11-08 | E-MTAB-11074 | biostudies-arrayexpress
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