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Lipid identification is a major bottleneck in high-throughput lipidomics studies. We combined the in silico fragmentation tool MetFrag with LipidMaps lipid-class specific classifiers which calculate probabilities for lipid class assignments. The LipidFrag approach was then applied to MS/MS spectra o...
2017-03-09 | MTBLS291 | MetaboLights
This experiment compares mRNA expression in cells with and without mitochondrial DNA (M-OM-^A+ and M-OM-^A0 cells respectively). We used global RNA profiling to measure mRNA expression in M-OM-^A+ and M-OM-^A0 cells harvested at log phase from synthetic medium with glucose (CM + 2% glucose, "CM...
ORGANISM(S): Saccharomyces cerevisiae 
Sphingolipids are important lipids and integral members of membranes, where they form small microdomains called lipid rafts. These rafts are enriched in cholesterol and sphingolipids, which influences biophysical properties. Interestingly, the membranes of the biomedical model organism Caenorhabditi...
2024-04-01 | MTBLS963 | MetaboLights

Introduction

Lipidomic profiling allows 100s if not 1000s of lipids in a sample to be detected and quantified. Modern lipidomics techniques are ultra-sensitive assays that enable the discovery of novel biomarkers in a variety of fields and provide new insight in mechanistic investigations. D...
2022-02-21 | MTBLS2016 | MetaboLights

The mechanisms by which macrophage metabolism is regulated and the effects of metabolism on diseases remain largely unknown. We show here that TGF-β regulates the glycolysis of macrophages independently of inflammatory cytokine production, and thus affects the survival in experimental sepsis. Spe...

2023-02-17 | MTBLS6886 | MetaboLights
Promoter-specific transcriptional activators (activators) stimulate transcription through direct interactions with one or more components of the transcription machinery, termed the “target”. Previous studies have provided evidence that the Tra1 subunit of the yeast SAGA (Spt-Ada-Gcn5-acetyltransfera...
ORGANISM(S): Saccharomyces cerevisiae 
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