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TranscriptomicsMicroarray analysis of vegetative phase change in maize
Vegetative phase change is the developmental transition from the juvenile phase to the adult phase during which a plant becomes competent for sexual reproduction. Gain of ability to flower is often accompanied by changes in patterns of differentiation in newly forming vegetative organs. In maize, ...
ORGANISM(S): Zea mays 
2010-06-20 | E-GEOD-9430 | biostudies-arrayexpress
TranscriptomicsGene Mapping via Bulked Segregant RNA-Seq (BSR-Seq)
Bulked segregant analysis (BSA) is an efficient method to rapidly and efficiently map genes responsible for mutant phenotypes. This procedure, however, requires access to quantitative genetic markers that are polymorphic in the mapping population. We have developed a modification of BSA (BSR-Seq) th...
ORGANISM(S): Zea mays 
2012-12-03 | E-MTAB-4234 | biostudies-arrayexpress
TranscriptomicsComplementation contributes to transcriptome complexity in maize (Zea mays L.) hybrids relative to their inbred parents
The aim of the present study was to globally survey the transcriptome of the two inbred lines B73 and Mo17 in comparison to their reciprocal F1-progeny in an unbiased approach via RNA-seq using four biological replicates to increase the power for detecting differential expression while maintaining a...
ORGANISM(S): Zea mays 
2012-06-27 | E-MTAB-4345 | biostudies-arrayexpress
TranscriptomicsContribution from Different Genomic Annotation Sets to Quantitative Trait Variation Revealed by Maize GWAS
The genomic distribution of trait-associated SNPs (TASs) discovered in genome-wide association studies (GWAS) can provide insight into the genetic architecture of complex traits and the design of future studies. Here we report on a maize GWAS that identified TASs underlying five quantitative traits ...
ORGANISM(S): Zea mays 
2012-06-11 | E-MTAB-4233 | biostudies-arrayexpress
TranscriptomicsGene Expression between two cell layers in maize shoot apical meristem
All above ground organs of higher plants are ultimately derived from shoot apical meristems (SAMs). The SAM exhibits distinctive structural organization, and monocot SAMs such as maize are comprised of two cell layers, a single cell layered tunica (L1) and a corpus (L2). Although recent research ha...
ORGANISM(S): Zea mays 
2013-01-28 | E-GEOD-16868 | biostudies-arrayexpress
TranscriptomicsDirect calibration of PICKY-designed microarrays
Picky is an optimal microarray design software which designed the NSF Rice 45K Array. A series of microarray experiments using a one-chip version of this array were conducted to validate as well as to develop a calibration method for Picky designed microarrays. Synthesized samples with known content...
ORGANISM(S): synthetic construct 
2010-06-21 | E-GEOD-14717 | biostudies-arrayexpress
TranscriptomicsExpression of genes and transposons in mop1 mutants and non-mutants
Transposable elements (TEs) comprise a substantial portion of many eukaryotic genomes and are typically transcriptionally silenced. RNA–dependent RNA polymerase 2 (RDR2) is a component of the RNA–directed DNA methylation (RdDM) silencing pathway. In maize, loss of mediator of paramutation1 (mop1) en...
ORGANISM(S): Zea mays 
2009-11-20 | E-GEOD-16789 | biostudies-arrayexpress
ProteomicsMYC2 phosphorylation
Mass spectrometry identification of MYC2 phosphorylation by the kinase Feronia
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2017-04-20 | MSV000080972 | MassIVE
TranscriptomicsRegulation of Gene Expression and Parent-of-Origin Effects in Maize Hybrids
Heterosis (hybrid vigor) refers to the superior performance of hybrid progeny relative to their parents. Although widely exploited in agriculture, the mechanisms responsible for heterosis are not well understood. As a monoecious organism, a given maize plant can be used as both male and female par...
ORGANISM(S): Zea mays 
2009-11-20 | E-GEOD-16136 | biostudies-arrayexpress
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