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MetabolomicsNon-targeted in vitro metabolomics for proteome-scale identification of novel enzymes in Escherichia coli
Many yet undiscovered enzymes still limit our understanding of metabolism. Here, we develop a high-throughput mass spectrometry approach to comprehensively profile proteome-scale protein collections for in vitro enzymatic activity. Overexpressed or purified proteins are incubated in a supplemented m...
2016-01-11 | MTBLS373 | MetaboLights
MetabolomicsMetabolome and proteome changes in skeletal muscle and blood of pre-weaning calves fed leucine and threonine supplemented diets

In pre-weaning calves, both leucine and threonine play important roles in growth and muscle metabolism. In this study, metabolomics, proteomics and clinical chemistry were used to assess the effects of leucine and threonine supplementation added to milk replacer on 14 newborn Holstein male calves...

2020-02-20 | MTBLS1045 | MetaboLights
ProteomicsUnderstanding early AutoAb signatures in Pituitary Adenomas using CDI HuProt™ (Human Proteome Microarray)
CDI HuProt™ (Human Proteome Microarray), was used to study Pituitary Adenomas PAs (Acromegaly, Cushing's and NFPA) using serum samples, from around 14 individuals (4 Healthy control, 4 Acromegaly, 3 Cushing’s and 3 NFPAs patient samples) were used study their autoantibody profiles. Patient serum sam...
ORGANISM(S): Homo sapiens 
2022-09-30 | E-MTAB-11947 | biostudies-arrayexpress
UnknownComparative Proteome Analysis in Schizosaccharomyces pombe Identifies Metabolic Targets to Improve Protein Production and Secretion.
Not available
S-EPMC5054337 | biostudies-literature
ProteomicsComparative Proteome Analysis in Schizosaccharomyces pombe identifies metabolic targets to improve protein production and secretion
Protein secretion in yeast is a complex process and its efficiency depends on a variety of parameters. We performed a comparative proteome analysis of a set of Schizosaccharomyces pombe strains producing the alpha-glucosidase maltase in increasing amounts to investigate the overall proteomic respons...
ORGANISM(S): Schizosaccharomyces pombe 
2016-08-08 | PXD002693 | Pride
Isobaric labeling 2d-lc maldi ms 5800 proteome analyzer High/low ph lc Itraq 
ProteomicsComparative Proteome Analysis in Schizosaccharomyces pombe identifies metabolic targets to improve protein production and secretion
This dataset contains additional experimental results for the determination of the effect of Fluconazole and amino acid supplementation to the NW10 strain of S. pombe. Protein secretion in yeast is a complex process and its efficiency depends on a variety of parameters. We performed a comparative p...
ORGANISM(S): Schizosaccharomyces pombe 
2016-08-08 | PXD003016 | Pride
Isobaric labeling 2d-lc maldi ms 5800 proteome analyzer High/low ph lc Itraq 
ProteomicsStoichiometry of the bacterial type III secretion export apparatus
Bacterial type III secretion systems are cell envelope-spanning effector protein-delivery machines essential for colonization and survival of many Gram-negative pathogens and symbionts. The membrane-embedded core unit of these secretion systems is termed needle complex. The needle complex is compose...
ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 
2016-02-25 | PXD003113 | Pride
Mass spectrometry Stoichiometry Salmonella Type iii secretion 
UnknownProteome of human minor salivary gland secretion.
Not available
S-EPMC2857507 | biostudies-literature
UnknownChoroid plexus apocrine secretion shapes CSF proteome during mouse brain development.
Not available
S-EPMC12229896 | biostudies-literature
ProteomicsTranscriptomic and phosphoproteomic profiling and metabolite analyses reveal the mechanism of NaHCO3-induced organic acid secretion in grapevine roots
Organic acid secretion is a widespread physiological response of plants to alkalinity. However, the features of alkali-induced secretion of organic acid and the underlying mechanism are poorly understood. Herein, it was indicated that oxalate was the main organic acid synthesized and secreted in vin...
ORGANISM(S): Vitis vinifera (Grape) 
2019-11-11 | PXD013746 | Pride
Transcriptome Organic acid secretion Phosphoproteome Grapevine Alkali stress 
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