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TranscriptomicsProbing the regulatory effects of specific mutations in three major binding domains of the pleiotropic regulator CcpA of Bacillus subtilis
Study the effect of single amino acid point mutations in CcpA at the transcriptome level. Cells were grown in LB with or without 1% glucose and harvested at an OD600 of 0.3 (early exponential phase). Bacillus subtilis strain ccpA::spec carrying pHT304 (empty plasmid), pWH_ccpA_M17R, pWH_ccpA_T62H, o...
ORGANISM(S): Bacillus subtilis 
2015-12-24 | E-GEOD-69575 | biostudies-arrayexpress
TranscriptomicsHigh- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis
In Bacillus subtilis and its relatives carbon catabolite control, a mechanism enabling to reach maximal efficiency of carbon and energy sources metabolism, is achieved by the global regulator CcpA (carbon catabolite protein A). CcpA in a complex with HPr-Ser-P (seryl-phosphorylated form of histidine...
ORGANISM(S): Bacillus subtilis 
2012-08-13 | E-GEOD-35154 | biostudies-arrayexpress
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