Sensory cortex - OmicsDI

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Single-Cell Extraction: For enzymatic digestion, pronase (Septomyces argeus, 1 mg/mL) was applied for 25 minutes at room temperature for P0 to P8 datasets. Cells were dissociated and triturated into a single-cell suspension in ACSF supplemented with 1% fetal calf serum (FCS) and DNase (1 µL/10 mL). Trituration was performed using three glass Pasteur pipettes of decreasing diameters. For P30 datasets, enzymatic digestion and cell dissociation were carried out using the Worthington Papain Dissociation System, following the manufacturer’s instructions. Single-Nuclei Extraction: For nuclei isolation, the Hibernate™-E Medium was removed, and 500 µL of chilled 0.1X NP40 Lysis Buffer was added. Tissue was homogenized using a Pellet Pestle (15 strokes) and incubated on ice for 5 minutes. The suspension was pipette-mixed 10 times using a wide-bore pipette tip and incubated for an additional 10 minutes on ice. Following lysis, 500 µL of chilled wash buffer was added, and the mixture was pipette-mixed 5 times using a regular-bore pipette tip. The suspension was filtered through a 30 µm cell strainer into a 50 mL tube to remove debris. The filtered suspension was transferred to a 1.5 mL tube and centrifuged at 950 × g for 10 minutes at 4°C. The supernatant was carefully removed, and the nuclei pellet was retained for downstream analysis. (2)
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CHEBI ID

CHEBI:71847 (1)
CHEBI:156118 (1)
CHEBI:71750 (1)
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CHEBI:156044 (1)
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CHEBI:66859 (1)
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CHEBI:66850 (1)

Metabolite Name

PE-NMe (44:4) (1)
PE-NMe(42:4) (1)
Phosphatidylinositol (P-36:5) (1)
Ganglioside GM3 (36:2) (1)
Phosphatidic acid (P-36:3) (1)
Cardiolipin 78:13(2-) (1)
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CL(79:2) (1)
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Phosphatidylethanolamine 32:1 zwitterion (1)
Phosphatidylethanolamine 42:2 (1)
PE-NMe(36:4) (1)
Phosphatidylglycerol 32:1(1-) (1)
CL(73:0) (1)
Phosphatidylserine 40:1(1-) (1)
Cardiolipin 78:14(2-) (1)
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PG(O-34:0) (1)
Phosphatidylethanolamine 42:3 zwitterion (1)
Cardiolipin 76:10(2-) (1)
Phosphatidylethanolamine 38:2 zwitterion (1)
PE(39:0) (1)
PE-NMe(42:1) (1)
CL(74:0) (1)
Cardiolipin 72:8(2-) (1)
Phosphatidylethanolamine O-36:1 zwitterion (1)
Phosphatidylethanolamine 38:4 (1)
CL(78:0) (1)
Phosphatidylethanolamine P-36:5 (1)

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Lab affiliation

Division of Cardiovascular Sciences, School of Medical Science, Faculty of Biology, Medicine and Health, The University of Manchester (5)
Department of Neuro- and Sensory Physiology University Medical Center Göttingen Humboldtallee 23 37073 Göttingen (3)
Institute of Clinical Pharmacology, Goethe-University, Frankfurt am Main, Germany (2)
Centre for Discovery Brain Sciences, University of Edinburgh, UK; Simons Initiative for the Developing Brain, University of Edinburgh, UK (1)
Radboud Institute for Molecular Life Sciences, Radboud University, Nijmegen - the Netherlands (1)
Chemistry Department, University of Illinois Urbana-Champaign (1)
Department of Neurology, Yale School of Medicine, Yale University, new haven CT, USA (1)
Division of Cardiovascular Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of Manchester (1)
Jösch Group "Neuroethology", ISTA (Institute of Science and Technology Austria), Am Campus 1, Klosterneuburg, Niederösterreich, Austria (1)
Division of Cardiovascular Science, School of Medical Sciences, Faculty of Biology Medicine and Health, The University of Manchester (1)
Laurent's Lab, Department of Biochemistry and Functional Genomics, Faculty of Medicine and Health Sciences, Cancer Research Institute (PRAC), Univeriste de Sherbrooke, Sherbrooke, QC, Canada (1)
The Francis Crick Institute (1)
Peking University (1)

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Transcription profiling of mouse P18, P24, P46, P104, and P46DR (dark reared) non-deprived vs. deprived visual cortex (GEO Super Series)

This SuperSeries is composed of the following subset Series:; GSE4263: P18 mouse non-deprived vs. deprived visual cortex; GSE4264: P24 mouse non-deprived vs. deprived visual cortex; GSE4265: P46 mouse non-deprived vs. deprived visual cortex; GSE4266: P104 mouse non-deprived vs. deprived visual corte...

ORGANISM(S): Mus musculus

2008-06-13 | E-GEOD-4269 | biostudies-arrayexpress

Transcription profiling of mouse P104 non-deprived vs. deprived visual cortex

Wild-type (C57Bl6) mice underwent monocular enucleation at P100 and were sacrificed 4 days later at P104. Bilateral (non-deprived and deprived) visual cortex was dissected from 1-mm thick coronal sections, and total RNA was extracted from the tissue lysate using Trizol (Gibco-BRL). Experiment Overal...

ORGANISM(S): Mus musculus

2008-06-13 | E-GEOD-4266 | biostudies-arrayexpress

Transcription profiling of mouse visual cortex from P46 mouse non-deprived vs. deprived

Wild-type (C57Bl6) mice underwent monocular enucleation at P42 and were sacrificed 4 days later at P46. Bilateral (non-deprived and deprived) visual cortex was dissected from 1-mm thick coronal sections, and total RNA was extracted from the tissue lysate using Trizol (Gibco-BRL). Experiment Overall ...

ORGANISM(S): Mus musculus

2008-06-13 | E-GEOD-4265 | biostudies-arrayexpress

Transcription profiling of mouse P24 animals non-deprived vs. deprived visual cortex

Wild-type (C57Bl6) mice underwent monocular enucleation at P20 and were sacrificed 4 days later at P24. Bilateral (non-deprived and deprived) visual cortex was dissected from 1-mm thick coronal sections, and total RNA was extracted from the tissue lysate using Trizol (Gibco-BRL). Experiment Overall ...

ORGANISM(S): Mus musculus

2008-06-13 | E-GEOD-4264 | biostudies-arrayexpress

Transcription profiling of mouse visual cortex from wild type animals sacrificed at P18 after mononuclear enucleation

Wild-type (C57Bl6) mice underwent monocular enucleation at P14 and were sacrificed 4 days later at P18. Bilateral (non-deprived and deprived) visual cortex was dissected from 1-mm thick coronal sections, and total RNA was extracted from the tissue lysate using Trizol (Gibco-BRL). Experiment Overall ...

ORGANISM(S): Mus musculus

2008-06-13 | E-GEOD-4263 | biostudies-arrayexpress

Transcription profiling of mouseP46DR dark reared mouse non-deprived vs. deprived visual cortex

Wild-type (C57Bl6) mice were dark reared from birth to P42, then underwent monocular enucleation at P42. They were then placed in a normal light-dark environment for 4 days, after which they were sacrificed (at P46). Visual cortex was dissected from 1-mm thick coronal sections, and total RNA was ext...

ORGANISM(S): Mus musculus

2008-06-13 | E-GEOD-4267 | biostudies-arrayexpress

Expression data of genes from the gustatory cortex follwing novel taste learning

Novel taste memories, critical for animal survival, are consolidated to form long term memories which are dependent on translation regulation in the gustatory cortex We used microarray for identification of genes involved in novel taste learning at two time points- 1 and 3 hours following memory for...

ORGANISM(S): Rattus norvegicus

2016-03-23 | E-GEOD-74546 | biostudies-arrayexpress

Proteomic landscape of the primary somatosensory cortex upon sensory deprivation

We employed label free quantitative mass spectrometry to address experience-dependent changes in the proteome after sensory deprivation in the primary somatosensory cortex. Cortical column- and layer-specific tissue samples were collected from control animals, with all whiskers intact, and animals w...

ORGANISM(S): Mus musculus (Mouse)

2017-08-24 | PXD005971 | Pride

RNA-seq of mouse primary somatosensory cortex (S1) in a neuropathic pain model after electroacupuncture stimulus

This study aims to identify the molecular mechanism in the sensory cortex underlying pain-relief effect of electroacupuncture

ORGANISM(S): Mus musculus

2021-01-09 | E-MTAB-9987 | biostudies-arrayexpress

Differential gene expression analysis in motor and sensory cortex as a result of experimental autoimmune encephalomyelitis (EAE), a neuroinflammatory model for Multiple sclerosis.

We performed micrarrays to investigate neuronal gene expression changes during acute inflammatory CNS axon injury using the murine myelin oligodendrocyte glycoprotein 35-55 (MOG35-55)-induced experimental autoimmune encephalomyelitis (EAE) model. The present study was assigned to assess the direct a...

ORGANISM(S): Mus musculus

2014-10-01 | E-GEOD-47900 | biostudies-arrayexpress

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