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TranscriptomicsMismatch oligonucleotides in human and yeast
Background Mismatched oligonucleotides are widely used on microarrays to differentiate specific from nonspecific hybridization. While many experiments rely on such oligos, the hybridization behavior of various degrees of mismatch (MM) structure has not been extensively studied. Here, we present the ...
ORGANISM(S): Homo sapiens 
2010-05-05 | E-GEOD-13175 | biostudies-arrayexpress
TranscriptomicsMismatch oligonucleotides in yeast
Tiled 10kb region centered around ACT1 gene (YFL039C, CHROMOSOME 6 @ coords 48760-59195), double-stranded, 36mers at 1bp spacing, with mismatches and deletions; also tiled 6 genes of interest (YBL092W, YGR155W, YOL040C, YOR312C, YMR242C, YLR229C), coding strand only, 36mers at 1bp spacing, with some...
ORGANISM(S): Saccharomyces cerevisiae 
2010-05-05 | E-GEOD-13174 | biostudies-arrayexpress
TranscriptomicsMismatch oligonucleotides in human
Tiled regions surrounding 5 human genes as 36mers, HBG2, TIMP3, SYN3, FLNA, FBX07. The first three of these genes, we tiled with various mismatch oligos in addition to 'perfect match' oligos. Keywords: Mismatch hybridization experiment Tiled perfect match and various designs of mismatch oligonucleo...
ORGANISM(S): Homo sapiens 
2010-05-05 | E-GEOD-13172 | biostudies-arrayexpress
TranscriptomicsDivergence of Transcription Factor Binding Sites in Related Yeast Species
A fundamental problem in biology is the molecular basis for divergence among related organisms. We have investigated the level of divergence of transcription factor binding sites for two key factors that regulate developmental processes in the budding yeasts. The genomic binding locations for the St...
ORGANISM(S): Saccharomyces mikatae 
2007-09-06 | E-GEOD-5421 | biostudies-arrayexpress
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