Serum-free medium

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CHEBI ID

CHEBI:30769 (17)
CHEBI:15428 (16)
CHEBI:32816 (15)
CHEBI:15741 (14)
CHEBI:26271 (13)
CHEBI:22660 (13)
CHEBI:6650 (13)
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CHEBI:27266 (10)
CHEBI:16704 (10)
CHEBI:15891 (10)
CHEBI:18237 (10)
CHEBI:18050 (10)
CHEBI:16856 (10)
CHEBI:16335 (10)
CHEBI:16919 (10)

Metabolite Name

Glycine (10)
Serine (7)
Adenine (7)
Creatine (7)
AMP (7)
Proline (6)
Malate (6)
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Choline (6)
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IMP (5)
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Phenylalanine (5)
Tyrosine (5)
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Lactate (5)
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IsPrivate

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2014 (166)
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2023 (33)
2020 (25)
2015 (24)
2018 (23)
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2017 (17)
2016 (15)
2013 (7)

Classification

bacteria (4)
fungi (1)
protozoa (1)

Modelling Approach

Constraint-based model (1)

Modification

4x(15)N labeled L-arginine (6)
4x(2)H labeled dimethylated residue (3)
2x(15)N labeled L-lysine (2)
4'-dihydroxyphenylalanine (1)
4-dinitrophenyl modified residue (1)
4-tetrahydrothiazine-3-carboxylic acid (1)

Study type

Transcription profiling by array (2494)
RNA-seq of coding RNA (419)
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CLIP-seq (5)
MicroRNA profiling by high throughput sequencing (4)
MNase-seq (3)

Release Date

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2005 (30)
2004 (11)
2003 (5)
1999 (2)
2002 (1)

Lab affiliation

University of Vienna, Faculty of Chemistry, Department of Analytical Chemistry (37)
The University of Melbourne (5)
Amsterdam UMC, Vrije Universiteit Amsterdam, Medical Oncology, Cancer Center Amsterdam, OncoProteomics Laboratory, Amsterdam, Netherlands (4)
Head of the Curie Institute Mass Spectrometry Platform (4)
Infection Program, Monash Biomedicine Discovery Institute, Department of Microbiology, Monash University, VIC 3800, Australia (4)
Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University, SE-751 23 Uppsala, Sweden. (4)
Zhejiang University, China (4)
1VIB-UGent Center for Medical Biotechnology, VIB, 9052 Ghent, Belgium 2Department of Biomolecular Medicine, Ghent University, 9052 Ghent, Belgium (3)
IMol Polish Academy of Sciences, Warsaw, Poland (3)
UCSF (3)
CRUK Beatson Institute (2)
Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands (2)
Department of Biochemistry UT Southwestern Medical Center (2)
Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany (2)
EPFL (2)
Functional Proteomics, ICR (2)
Head of Drug Discovery and Evaluation Charles University in Prague, BIOCEV Průmyslová 595 252 50 Vestec Czech Republic (2)
IMol Institute, Polish Academy of Sciences, Warsaw, Poland (2)
Institute for Clinical Biochemistry and Pathobiochemistry, German Diabetes Center (DDZ), Leibniz Center for Diabetes Research at Heinrich-Heine-University Düsseldorf, Auf´m Hennekamp 65, 40225 Düsseldorf, Germany (2)
Laboratory of Systems Biology, Institute of Biomedical Chemistry, Moscow (2)
Nanyang Technological University (2)
National Heart and Lung Institute, Imperial College London, United Kingdom (2)
OncoProteomics Laboratory, Dept of Medical Oncology, VU University Medical Center, Amsterdam, The Netherlands (2)
Proteomics group, Ri.MED Foundation, Palermo, Italy (2)
Southern University of Science and Technology (2)
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China (2)
Unit of Proteomics- Centro Cardiologico Monzino IRCCS (2)
University of Oxford (2)
Université de Lille, Sciences et Technologies - INSERM U1192 - Laboratoire PRISM, France (2)
Uppsala University, Department of Chemistry-BMC, Analytical Chemistry (2)

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Label-free shotgun proteomics of Mycoplasma pneumoniae grown in serum-free medium

We developed a serum and animal component-free medium capable of supporting robust growth of the genome-reduced bacterium Mycoplasma pneumoniae. Here, we analyzed the proteome of the wild-type strain grown in serum-free vB13 medium compared to rich medium (Hayflick medium).

ORGANISM(S): Mycoplasma pneumoniae (strain ATCC 29342 / M129) Bacteria

2023-04-05 | PXD037431 | Pride

Mycoplasma pneumoniae Serum-free medium

Gene expression in human keratinocytes cultivated with serum-free medium and serum containing medium

PRJNA761384 | ENA

Human umbilical cord blood-derived endothelial colony forming cells under serum-free conditions

Derivation and expansion of human umbilical cord blood-derived endothelial colony forming cells under serum-free conditions - a transcriptome analysis. Endothelial colony forming cells (ECFCs) were isolated from term umbilical cord blood units. ECFCs were expanded under standard, fetal bovine serum ...

ORGANISM(S): Homo sapiens

2010-07-29 | E-GEOD-20504 | biostudies-arrayexpress

RNA-seq of Mycoplasma pneumoniae grown in serum-free medium

PRJEB56809 | ENA

RNA-seq of Mycoplasma pneumoniae grown in serum-free medium

We developed a serum and animal component-free medium capable of supporting robust growth of the genome-reduced bacterium Mycoplasma pneumoniae. Here, we performed RNA-seq expression analysis of the wild-type strain grown in serum-free vB13 medium.

ORGANISM(S): Mycoplasma pneumoniae

2023-04-03 | E-MTAB-12308 | biostudies-arrayexpress

Epigenome and transcriptome of naive pluripotent mouse embryonic stem (ES) cells cultured in 2i serum-free medium

This study describes the epigenetic and transcriptome profiling of mouse ES cells cultures in 2i serum-free medium. The proteins for which ChIP-Seq profiles were generated in these cells, as well as for cells grown in serum conditions, include H3K4me3, H3K36me3, H3K27me3, H3K9me3, Suz12 and Ezh2. A...

ORGANISM(S): Mus musculus

2012-04-26 | E-GEOD-23943 | biostudies-arrayexpress

Gene Expression Profile of Human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) Culutured in Chemical Defined Serum Free Medium

Human umbilical cord mesenchymal stem cells maintained multipotency and immunosuppressive ability when being cultured in chemical defined serum free medium, but gained different gene expression profile. We used microarrays to identify the transcriptional difference between human umbilical cord mesen...

ORGANISM(S): Homo sapiens

2014-10-28 | E-GEOD-62665 | biostudies-arrayexpress

Gene expression in human keratinocytes cultivated with serum-free medium and serum containing medium

The objective of this study was thus to develop ab initio that chemically defined serum-free medium. We used a statistical design of experiment (DOE) approach to screen a large number of candidate factors, for both their potential individual and synergetic effects, as to identify a formulation which...

ORGANISM(S): Homo sapiens

2021-09-08 | GSE183594 | GEO

Characterization of human dental pulp cells grown in chemically defined serum-free medium

Dental pulp cells obtained from several donors proliferated actively in a serum-free medium STK2. The growth rate of dental pulp cells from most donors was higher in the serum-free medium than that in a medium containing 10% serum. DNA microarray analyses showed that gene expression profile of denta...

ORGANISM(S): Homo sapiens

2018-03-14 | GSE97199 | GEO

Variations of secretome profiles according to conditioned medium preparation: the example of human mesenchymal stem cell derived adipocytes

One challenging point in analysing cellular secretome collected as conditioned medium is cross contamination by cell culture media components, especially bovine serum proteins. A common approach for serum removal is to wash the cells, an alternative is to grow cells using serum-free conditions. Give...

ORGANISM(S): Homo sapiens (Human)

2015-07-03 | PXD002217 | Pride

Adipocyte/conditioned medium/mesenchymal stem cell /secretome

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