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TranscriptomicsTranscription profiling of mouse IgM+IgD+ cells and srf-deficient IgM+IgD+ cells isolated from spleens to investigate the role of serum response factor
Serum response factor (SRF), a MADS-box transcription factor, is essential for murine embryonic development and for the function of muscle cells and neurons. SRF and its transcriptional co-factors are broadly expressed. To determine the in vivo role of SRF in developing lymphocytes we specifically i...
ORGANISM(S): Mus musculus 
2008-06-15 | E-GEOD-7412 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse SRF-null or control cardiomyocytes to study roles of the serum response factor in regulating contractile apparatus gene expression.
Serum Response Factor (SRF) is a transcriptional regulator required for mesodermal development. Numerous studies have implicated SRF as a central regulator of muscle gene expression and myogenesis. In this present study we used a loss of function approach to delineate the role of SRF in cardiac myoc...
ORGANISM(S): Mus musculus 
2007-09-07 | E-GEOD-3181 | biostudies-arrayexpress
TranscriptomicsMx-Cre mediated deletion of SRF in murine hematopoietic LSK (Lin-Sca1+c-Kit+) cells
Analysis of hematopoietic LSK(Lin-Sca1+c-Kit+) cells lacking the Serum response factor (SRF) gene. Results provide insight into the role of SRF in regulating genetic programs important for hematopoietic stem cell development Comparison of the gene expression profile between murine Lin-Sca1+c-Kit+ ce...
ORGANISM(S): Mus musculus 
2010-08-12 | E-GEOD-23556 | biostudies-arrayexpress
TranscriptomicsKey roles for SRF in embryonic epidermal differentiation
During development, a polarized sheet of epidermal cells undergoes stratification and differentiation to produce the skin barrier. Through mechanisms poorly understood, the process involves adhesion and Notch signaling. To elucidate how epidermal embryogenesis is governed, we conditionally targeted ...
ORGANISM(S): Mus musculus 
2011-01-20 | E-GEOD-25548 | biostudies-arrayexpress
TranscriptomicsHeart specific moderate over-expression of serum response factor (SRF) in 6mo mouse.
To identify in vivo new cardiac SRF target genes and to study the response of these novel genes to SRF overexpression, we employed a cardiac-specific, transgenic mouse model that has a phenotype in young adulthood which resembles that of the typically aged heart. Using this “cardiac aging” model, we...
ORGANISM(S): Mus musculus 
2011-08-02 | E-GEOD-19874 | biostudies-arrayexpress
TranscriptomicsSRF Overexpression Leads to Altered Expression of MicroRNAs and Contributes to Cardiac Hypertrophy
The role of SRF in the regulation of microRNA expression and microRNA biogenesis in cardiac hypertrophy has not been well established. In this report, we employed a distinct transgenic mouse model to study the impact of SRF on cardiac microRNA expression and microRNA biogenesis. Cardiac-specific ove...
ORGANISM(S): Mus musculus 
2011-02-16 | E-GEOD-23044 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse embryonic stem (ES) cells overexpressing serum response factor SRF to identify potential transcriptional targets of SRF
Empty vector, SRF-dM-VP16 (control construct lacking the SRF DNA binding domain), or SRF-VP16 were expressed in wild-type ES cells (E14wt), SRF heterozygotes (E99-/+) or two SRF ko cell lines (E81-/-,E100-/-). RNA was harvested 72h after transfection. Each condition was performed in duplicate, excep...
ORGANISM(S): Mus musculus 
2007-11-06 | E-GEOD-1948 | biostudies-arrayexpress
TranscriptomicsMRTF-SRF signaling is required for seeding of HSC/Ps in bone marrow during development
Chemokine signaling is important for the seeding of different sites by hematopoietic stem cells during development. Serum Response Factor (SRF) controls multiple genes governing adhesion and migration, mainly by recruiting members of the Myocardin-Related Transcription Factor (MRTF) family of G-acti...
ORGANISM(S): Mus musculus 
2015-03-04 | E-GEOD-63820 | biostudies-arrayexpress
TranscriptomicsPF4-Cre mediated deletion of SRF in murine megakaryocytes.
Srf is a MADS-box transcription factor that is critical for muscle differentiation. Its function in hematopoiesis has not yet been revealed. Mkl1, a cofactor of Srf, is part of the t(1;22) translocation in acute megakaryoblastic leukemia, and plays a critical role in megakaryopoiesis. In order to te...
ORGANISM(S): Mus musculus 
2010-07-13 | E-GEOD-21859 | biostudies-arrayexpress
TranscriptomicsIdentification of SRF binding sites in Three Human Cell types
We used chromatin immunoprecipitation (ChIP) in combination with human promoter microarrays to identify 216 putative SRF binding sites in the human genome. We performed independent quantitative PCR validation at over half of these sites that resulted in 146 sites we assert to be true binding sites ...
ORGANISM(S): Homo sapiens 
2010-06-10 | E-GEOD-5998 | biostudies-arrayexpress
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